ArticlePLoS genetics2019
Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm.
Article in PLoS genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed.
- Review
- Comparative single-nucleus transcriptomics reveals asymmetric evolution of the Drosophila male and female germlines.PLoS biology · 2026Article
- Pervasive convergent evolution of sperm conjugation across the Arthropoda tree of life.Nature communications · 2026Review
- A Testis-Specific Aralkylamine N-Acetyltransferase Regulates Dimorphic Sperm Function and Male Fertility in Moths.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- Proteomic analysis of dimorphic sperm in the cabbage white butterfly,Frontiers in insect science · 2026Article
- Analysis of Transcript Expression and Core Promoter DNA Sequences of Brain, Adipose Tissues and Testis in Human and Fruit Fly.International journal of molecular sciences · 2025Article
- The physical consequences of sperm gigantism.bioRxiv : the preprint server for biology · 2025Article
- Dichotomous transcriptome divergence at the cell-type level between thebioRxiv : the preprint server for biology · 2025Article
- Multi-omics integration reveals Vha68-3 as a testicular aging-specific factor that coordinates spermatid elongation through mitochondrial metabolic homeostasis.Cellular & molecular biology letters · 2025Article
- A deficiency screen identifies genomic regions critical for sperm head-tail connection.G3 (Bethesda, Md.) · 2025Article
- Versatile gamma-tubulin complexes contribute to the dynamic organization of MTOCs during Drosophila spermatogenesis.Communications biology · 2024Article
- A deficiency screen identifies genomic regions critical for sperm head-tail connection.bioRxiv : the preprint server for biology · 2024Article
- Deficiency ofInternational journal of molecular sciences · 2024Article
- Proteomic diversification of spermatostyles among six species of whirligig beetles.Molecular reproduction and development · 2024Article
- Mitochondrial Differentiation during Spermatogenesis: Lessons fromInternational journal of molecular sciences · 2024Review
- Leucine aminopeptidase1 controls egg deposition and hatchability in male Aedes aegypti mosquitoes.Nature communications · 2024Article
- Structure and molecular basis of spermatid elongation in theOpen biology · 2023Review
- Metabolic regulation of proteome stability via N-terminal acetylation controls male germline stem cell differentiation and reproduction.Nature communications · 2023Article
- Single-cell RNA sequencing reveals cell landscape following antimony exposure during spermatogenesis in Drosophila testes.Cell death discovery · 2023Article
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9 authors.
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Abstract
Drosophila melanogaster sperm reach an extraordinary long size, 1.8 mm, by the end of spermatogenesis. The mitochondrial derivatives run along the entire flagellum and provide structural rigidity for flagellar movement, but its precise function and organization is incompletely understood. The two mitochondrial derivatives differentiate and by the end of spermatogenesis the minor one reduces its size and the major one accumulates paracrystalline material inside it. The molecular constituents and precise function of the paracrystalline material have not yet been revealed. Here we purified the paracrystalline material from mature sperm and identified by mass spectrometry Sperm-Leucylaminopeptidase (S-Lap) family members as important constituents of it. To study the function of S-Lap proteins we show the characterization of classical mutants and RNAi lines affecting of the S-Lap genes and the analysis of their mutant phenotypes. We show that the male sterile phenotype of the S-Lap mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives. Our work shows that S-Lap proteins localize and accumulate in the paracrystalline material of the major mitochondrial derivative. Therefore, we propose that S-Lap proteins are important constituents of the paracrystalline material of Drosophila melanogaster sperm.
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