Evidence map›Paper›PMID 30802005›Full record

ArticleCurrent protocols in microbiology2019

An Automated Assay to Measure Phagocytosis of Cryptococcus neoformans.

Andrew L Chang, Camaron R Hole, Tamara L Doering

Open access · greenAbstract read
In one paragraph

Article in Current protocols in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. A Quick reCAP: DiscoveringJournal of fungi (Basel, Switzerland) · 2024
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Andrew L ChangWashington University in St. Louis, St. Louis, Missouri.
Camaron R HoleWashington University in St. Louis, St. Louis, Missouri.
Tamara L DoeringWashington University in St. Louis, St. Louis, Missouri.
Washington University in St. Louis · US

Funding

HOST FACTORS IN CRYPTOCOCCAL PATHOGENESISR01AI102882 · NIAID · WASHINGTON UNIVERSITY · PI DOERING, TAMARA L · 2013 to 2017
$2.2M
Cryptococcal factors in host cell interactionsR21AI140979 · NIAID · WASHINGTON UNIVERSITY · PI DOERING, TAMARA L · 2018 to 2019
$428k
NIAID NIH HHS R01 AI102882NIAID NIH HHS R21 AI140979
6 · The paper itself

Abstract

Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningoencephalitis, which kills 200,000 individuals worldwide each year. It is ubiquitous in the environment and is first inhaled into the lungs of the host, where it is taken up by phagocytes. The interaction of these fungal cells with host phagocytes, therefore, is a critical step in the pathogenesis of this disease. One characteristic of this initial step in host-pathogen interactions is the avidity with which fungal cells are taken up by phagocytes, described by the phagocytic index. In this chapter, we detail a high-throughput method of directly assessing the phagocytic index of fungal cells using an imaging-based paradigm. By automating image collection and processing, this method permits rapid assessment of this critical host interaction. © 2019 by John Wiley & Sons, Inc.

Indexed as

PhagocytosisAutomationCell LineColony Count, MicrobialCryptococcosisCryptococcus neoformansHumansMicroscopyPhagocytesStaining and LabelingautomationCryptococcus neoformansphagocytic indexphagocytosis

Identifiers

PMID30802005
PMCPMC6586510
OpenAlexW2916265770

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.