ArticleTransboundary and emerging diseases2019
Point-of-care diagnostic assay for rapid detection of porcine deltacoronavirus using the recombinase polymerase amplification method.
Article in Transboundary and emerging diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
20 citing papers in PubMed.
- Thermally Responsive Alkane Partitions and a Magnetofluidic Assay for Point-of-Sample Detection of Viruses in Wastewater.Biosensors · 2025Article
- Development of a visual detection method of porcine deltacoronavirus using loop-mediated isothermal amplification.Frontiers in microbiology · 2024Article
- The establishment of a recombinase polymerase amplification technique for the detection of mouse poxvirus.BMC veterinary research · 2023Article
- Rapid detection of goose astrovirus genotypes 2 using real-time reverse transcription recombinase polymerase amplification.BMC veterinary research · 2023Article
- Emergent Molecular Techniques Applied to the Detection of Porcine Viruses.Veterinary sciences · 2023Review
- Establishment of a recombinase polymerase amplification detection method for Puccinia striiformis f. sp. tritici.Scientific reports · 2023Article
- Development of an RT-PCR-based RspCas13d system to detect porcine deltacoronavirus.Applied microbiology and biotechnology · 2023Article
- An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a.Foods (Basel, Switzerland) · 2023Article
- Real-time detection of Seneca Valley virus by one-tube RPA-CRISPR/Cas12a assay.Frontiers in cellular and infection microbiology · 2023Article
- Visual detection of porcine epidemic diarrhea virus by recombinase polymerase amplification combined with lateral flow dipstrip.BMC veterinary research · 2022Article
- A Novel, Cleaved Probe-Based Reverse Transcription Loop-Mediated Isothermal Amplification Method for Specific and Sensitive Detection of Porcine Deltacoronavirus.Frontiers in veterinary science · 2022Article
- Porcine Enteric Coronavirus Infections in Wild Boar in Poland - a Pilot Study.Journal of veterinary research · 2021Article
- A critical review of point-of-care diagnostic technologies to combat viral pandemics.Analytica chimica acta · 2021Review
- Rapid and Sensitive Detection ofFrontiers in microbiology · 2021Article
- A rapid field-based assay using recombinase polymerase amplification for identification ofJournal of pest science · 2021Article
- Tandem Mass Tag-Based Quantitative Proteome Analysis of Porcine Deltacoronavirus (PDCoV)-Infected LLC Porcine Kidney Cells.ACS omega · 2020Article
- Microfluidic-RT-LAMP chip for the point-of-care detection of emerging and re-emerging enteric coronaviruses in swine.Analytica chimica acta · 2020Article
- Rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstick.BMC veterinary research · 2020Article
- Establishment of a Real-Time Recombinase Polymerase Amplification Assay for the Detection of Avian Reovirus.Frontiers in veterinary science · 2020Article
- Point-of-care diagnostic assay for rapid detection of porcine deltacoronavirus using the recombinase polymerase amplification method.Transboundary and emerging diseases · 2019Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Porcine deltacoronavirus (PDCoV) has emerged and spread throughout the porcine industry in many countries over the last 6 years. PDCoV caused watery diarrhoea, vomiting and dehydration in newborn piglets. A sensitive diagnostic method would be beneficial to the prevention and control of PDCoV infection. Recombinase polymerase amplification (RPA) is an isothermal amplification method which has been widely used for virus detection. A probe-based reverse transcription RPA (RT-RPA) assay was developed for real-time detection of PDCoV. The amplification can be finished in 20 min and fluorescence monitoring was performed by a portable device. The lowest detection limit of the PDCoV RT-RPA assay was 100 copies of RNA molecules per reaction; moreover, the RT-RPA assay had no cross-reaction with other common swine viruses. The clinical performance of the RT-RPA assay was evaluated using 108 clinical samples (54 intestine specimens and 54 faecal swab specimens). The coincidence rate of the detection results for clinical samples between RT-RPA and RT-qPCR was 97.2%. In summary, the real-time RT-RPA assay offers a promising alternative to RT-qPCR for point-of-care detection of PDCoV.
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