Evidence map›Paper›PMID 30801358›Full record

ArticleAnesthesia and analgesia2019

Suppression of Human Natural Killer Cells by Different Classes of Opioids.

Dermot P Maher, Deepa Walia, Nicola M Heller

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Anesthesia and analgesia, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 3 pooled it
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 3 syntheses or guidelines pooled it, 55 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Dermot P MaherFrom the Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Hospital, Baltimore, Maryland.
Deepa Walia
Nicola M Heller
Johns Hopkins Hospital · US

Funding

Program in Postdoctoral Research Training in AnesthesiologyT32GM075774 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI SAPNA R KUDCHADKAR, Emmett E Whitaker · 2006 to 2026
$8.1M
Sex differences in macrophage differentiation in allergic lung inflammationR01HL124477 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI HELLER, NICOLA M · 2014 to 2018
$2.0M
NHLBI NIH HHS R01 HL124477NIGMS NIH HHS T32 GM075774
6 · The paper itself

Abstract

backgroundThe use of regional and other opioid-sparing forms of anesthesia has been associated with a decrease in the recurrence of certain malignancies. Direct suppression of human natural killer cells by opioids has been postulated to explain this observation. However, the effect of different classes of opioids on suppression of natural killer cell cytotoxicity has not been systematically characterized.

methodsAfter confirming that freshly isolated natural killer cells from peripheral human blood express opioid receptors, cells were incubated with increasing concentrations of clinically used or receptor-specific opioid agonists. We also evaluated the effect of pretreatment with receptor-specific antagonists or naloxone. Treated natural killer cells were then coincubated with a carboxyfluorescein succinimidyl ester-labeled target tumor cell line, K562. Annexin V staining was used to compare the percent of tumor cell apoptosis in the presence of opioid-pretreated and untreated natural killer cells. Treated samples were compared to untreated samples using Kruskal-Wallis tests with a post hoc Dunn correction.

resultsMorphine, methadone, buprenorphine, loperamide, [D-Ala2, N-MePhe4, Gly-ol]-enkephalin, and U-50488 significantly decreased natural killer cell cytotoxicity. When natural killer cells were pretreated with naloxone, cyprodime, and nor-binaltorphimine before exposure to morphine, there was no difference in natural killer cytotoxicity, compared to the amount observed by untreated natural killer cells. Fentanyl, O-desmethyltramadol, and [D-Pen2,D-Pen5] enkephalin did not change natural killer cell cytotoxicity compare to untreated natural killer cells.

conclusionsIncubation of isolated natural killer cells with certain opioids causes a decrease in activity that is not observed after naloxone pretreatment. Suppression of natural killer cell cytotoxicity was observed with μ- and κ-receptor agonists but not δ-receptor agonists. These data suggest that the effect is mediated by μ- and κ-receptor agonism and that suppression is similar with many clinically used opioids.

Indexed as

3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-IsomerAnalgesics, OpioidAnesthesiaBuprenorphineEnkephalin, D-Penicillamine (2,5)-FentanylFluoresceinsHumansImmunosuppression TherapyK562 CellsKiller Cells, NaturalLoperamideMethadoneMorphinansMorphineNaloxone3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer5-(6)-carboxyfluorescein diacetate succinimidyl esterAnalgesics, OpioidBuprenorphinecyprodimeEnkephalin, D-Penicillamine (2,5)-FentanylFluoresceinsLoperamideMethadoneMorphinansMorphineNaloxoneNaltrexonenorbinaltorphimineO-demethyltramadolSuccinimidesTLR4 protein, humanToll-Like Receptor 4Tramadol

Identifiers

PMID30801358
PMCPMC6726115
OpenAlexW2919799243

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.