ArticlemSystems
Ultrahigh-Throughput Multiplexing and Sequencing of >500-Base-Pair Amplicon Regions on the Illumina HiSeq 2500 Platform.
Article in mSystems. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
101 citing papers in PubMed.
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- Influence of Hormonal Contraceptive Use and HIV on Cervicovaginal Cytokines and Microbiota in Malawi.mSphere · 2023Trial
- Vaginal Microbiota and Mucosal Pharmacokinetics of Tenofovir in Healthy Women Using a 90-Day Tenofovir/Levonorgestrel Vaginal Ring.Frontiers in cellular and infection microbiology · 2022Trial
- Trial
- Early-Life Dietary Protein Source Reshapes Gut Microbiota but Fails to Curtail Diet-Induced Metabolic Syndrome in Young Rats.Obesity (Silver Spring, Md.) · 2026Article
- Evaluating the Impact of Little Cigar Use on the Oral Bacterial Microbiota of Cigarette Smokers.Pathogens (Basel, Switzerland) · 2026Article
- Comparing Asymptomatic Versus Symptomatic Bacterial Vaginosis: Clinical Characteristics, Vaginal Microbiota, and Lactic Acid Profiles.Sexually transmitted diseases · 2026Article
- CUPID-seq enables highly multiplexed amplicon sequencing via combinatorial in-line dual indexing.bioRxiv : the preprint server for biology · 2026Article
- Microbial assembly and metabolic restructuring following COAMB Express · 2026Article
- Assessing the impact of phage therapy on growth performance, microbiome and phage specific immune response in chickens.Scientific reports · 2026Article
- Glucosamine Yield Improvement in EngineeredFoods (Basel, Switzerland) · 2026Article
- Impact of maternal HIV infection on the gut microbiome and metabolome of mothers and infants: the PRACHITi cohort in Pune, India.Nature communications · 2026Article
- The Nugent score is an inappropriate diagnostic tool for neovaginal bacteria in transfeminine people.Communications medicine · 2026Article
- Resource landscape shapes the composition and stability of the human vaginal microbiota.PLoS biology · 2026Article
- Microbial communities associated with two populations of the spongeBiodiversity data journal · 2026Article
- The complete chloroplast genome sequence and phylogenetic analysis ofMitochondrial DNA. Part B, Resources · 2026Article
- The vaginal microbiota, symptoms, and local immune correlates in transmasculine individuals using sustained testosterone therapy.Cell reports · 2025Article
- The neovaginal microbiota, symptoms, and local immune correlates in transfeminine individuals with penile inversion vaginoplasty.Cell reports · 2025Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
Abstract
Amplification, sequencing, and analysis of the 16S rRNA gene affords characterization of microbial community composition. As this tool has become more popular and amplicon-sequencing applications have grown in the total number of samples, growth in sample multiplexing is becoming necessary while maintaining high sequence quality and sequencing depth. Here, modifications to the Illumina HiSeq 2500 platform are described which produce greater multiplexing capabilities and 300-bp paired-end reads of higher quality than those produced by the current Illumina MiSeq platform. To improve the feasibility and flexibility of this method, a 2-step PCR amplification protocol is also described that allows for targeting of different amplicon regions, and enhances amplification success from samples with low bacterial bioburden.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.