ArticleBiomedical optics express2019
Light-sheet microscopy with length-adaptive Bessel beams.
Article in Biomedical optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 33 citations in OpenAlex.
- Interferometric modulation for generating extended light sheet: improving field of view.Journal of biomedical optics · 2024Article
- Selective plane illumination microscope dedicated to volumetric imaging in microfluidic chambers.Biomedical optics express · 2022Article
- Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination.Nature communications · 2021Article
- Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy.Scientific reports · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In light-sheet microscopy, a confined layer in the focal plane of the detection objective is illuminated from the side. The illumination light-sheet usually has a constant beam length independent of the shape of the biological object. Since the thickness and the length of the illumination light-sheet are coupled, a tradeoff between resolution, contrast and field of view has to be accepted. Here we show that scanned Bessel beams enable object adapted tailoring of the light-sheet defined by its beam length and position. The individual beam parameters are obtained from automatic object shape estimation by low-power laser light scattered at the object. Using Arabidopsis root tips, cell clusters and zebrafish tails, we demonstrate that Bessel beam light-sheet tailoring leads to a 50% increase in image contrast and a 50% reduction in photobleaching. Light-sheet tailoring requires only binary amplitude modulation, therefore allowing a real time illumination adaptation with little technical effort in the future.
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Registered trials
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