ArticleRoyal Society open science2019
Identification of copper (Cu) stress-responsive grapevine microRNAs and their target genes by high-throughput sequencing.
Article in Royal Society open science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Limitations of Single Prediction Tools in miRNA Profiling of Grapevine Viral Coinfection.Genes · 2026Article
- Root-Specific Signal Modules Mediating Abiotic Stress Tolerance in Fruit Crops.Plants (Basel, Switzerland) · 2026Review
- Genome-wide analysis of Phaseolus vulgaris L. miR172 gene family members under heavy metal stress.BMC genomics · 2026Article
- Identification and Characterization of Copper-Responsive miRNAs and Their Target Genes in Jerusalem Artichoke.Plants (Basel, Switzerland) · 2025Article
- MicroRNA gatekeepers: Orchestrating rhizospheric dynamics.Journal of integrative plant biology · 2025Review
- The grapevine miR827a regulates the synthesis of stilbenes by targeting VqMYB14 and gives rise to susceptibility in plant immunity.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- microRNAs: Key Players in Plant Response to Metal Toxicity.International journal of molecular sciences · 2022Review
- MicroRNA Mediated Plant Responses to Nutrient Stress.International journal of molecular sciences · 2022Review
- Small RNA profiling reveals the involvement of microRNA-mediated gene regulation in response to symbiosis in raspberry.Frontiers in microbiology · 2022Article
- Comparative analysis identifies micro-RNA associated with nutrient homeostasis, development and stress response in Arabidopsis thaliana upon high Zn and metal hyperaccumulator Arabidopsis halleri.Physiologia plantarum · 2021Article
- Role of miRNAs in the host-pathogen interaction between sugarcane and Colletotrichum falcatum, the red rot pathogen.Plant cell reports · 2021Article
- Genome-Wide Identification of Copper Stress-Regulated and Novel MicroRNAs in Mulberry Leaf.Biochemical genetics · 2021Article
- Identification of Known and Novel MicroRNAs in Raspberry Organs Through High-Throughput Sequencing.Frontiers in plant science · 2020Article
- Identification of microRNAs and relative target genes in Moringa oleifera leaf and callus.Scientific reports · 2019Article
- MicroRNA-124: An emerging therapeutic target in cancer.Cancer medicine · 2019Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are a class of single-stranded non-coding small RNAs (sRNAs) that are 20-24 nucleotides (nt) in length. Extensive studies have indicated that miRNAs play important roles in plant growth, development and stress responses. With more copper (Cu) and copper containing compounds used as bactericides and fungicides in plants, Cu stress has become one of the serious environmental problems that affect plant growth and development. In order to uncover the hidden response mechanisms of Cu stress, two small RNA libraries were constructed from Cu-treated and water-treated (Control) leaves of 'Summer Black' grapevine. Following high-throughput sequencing and filtering, a total of 158 known and 98 putative novel miRNAs were identified in the two libraries. Among these, 100 known and 47 novel miRNAs were identified as differentially expressed under Cu stress. Subsequently, the expression patterns of nine Cu-responsive miRNAs were validated by quantitative real-time PCR (qRT-PCR). There existed some consistency in expression levels of Cu-responsive miRNAs between high throughput sequencing and qRT-PCR assays. The targets prediction of miRNAs indicates that miRNA may regulate some transcription factors, including AP2, SBP, NAC, MYB and ARF during Cu stress. The target genes for two known and two novel miRNAs showed specific cleavage sites at the 10th and/or 11th nucleotide from the 5'-end of the miRNA corresponding to their miRNA complementary sequences. The findings will lay the foundation for exploring the role of the regulation of miRNAs in response to Cu stress and provide valuable gene information for breeding some Cu-tolerant grapevine cultivars.
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