Development and Comparison of In-house Line Probe Assay (LiPA) and SYBR Green Real-time PCR Regarding the Detection of Periodontal Pathogens.

Mohammad Soleimani, Mohammad Reza Zolfaghari

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Article in Avicenna journal of medical biotechnology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 citing paper in PubMed.

Quantitative Assessment of Primary Colonizer Adhesion on Different Resin-Based Restorative Materials Using SYBR Green qPCR.Dentistry journal · 2026
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2 authors.

Mohammad SoleimaniDepartment of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.

Mohammad Reza ZolfaghariDepartment of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPeriodontal disease, which can become a chronic condition, is an inflammatory disease that upsets the soft and hard structures supporting the teeth. The aim of the present study was to design and develop an in-house Line Probe Assay (LiPA), to detect putative periodontitis-related bacterial pathogens, and compare it with SYBR Green Real-time PCR.

methodsThe LiPA method was launched using biotinylated 16s rRNA universal primers and specific probes for each of the five bacteria including

resultsThe detection limit of the LiPA was 2760 copies of targeted genes. In testing analytical specificity, only signals corresponding to the specific biotinylated products were produced. The calculated diagnostic sensitivity of the LiPA for the five bacterial targets ranged from 96.4 to 100%, whereas the diagnostic specificity was between 90.9 and 100%. Comparing the results, no noticeable difference (p=0.4795) was observed between the two methods.

conclusionTo screen periodontal pathogens, a simple, inexpensive and accurate method is desirable. The in-house LiPA, having advantages such as high specificity and sensitivity, and the ability to detect five major periodontal pathogens, offers the option of evaluating samples without the need for a post-PCR platform.

Indexed as

PeriodontitisReal-time PCRSensitivity and specificity

Identifiers

PMID30800247

PMCPMC6359689

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