ArticleFrontiers in neuroanatomy2019
Flexible Multi-Beam Light-Sheet Fluorescence Microscope for Live Imaging Without Striping Artifacts.
Article in Frontiers in neuroanatomy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Beyond Membrane Potential: Exploiting Signal Complexity in Genetically Encoded Voltage Indicators.Sensors (Basel, Switzerland) · 2026Article
- Voltage imaging in zebrafish using high-speed light-sheet microscopy.Neurophotonics · 2026Review
- Video microscopy: an old story with a bright biological future.Biomedical engineering online · 2025Review
- Imaging Approaches to Investigate Pathophysiological Mechanisms of Brain Disease in Zebrafish.International journal of molecular sciences · 2023Review
- Compact, high-speed multi-directional selective plane illumination microscopy.Biomedical optics express · 2023Article
- 3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy.Communications biology · 2022Article
- Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy.Biomedical optics express · 2022Article
- Non-telecentric two-photon microscopy for 3D random access mesoscale imaging.Nature communications · 2022Article
- Identification of microplastics using 4-dimethylamino-4'-nitrostilbene solvatochromic fluorescence.Microscopy research and technique · 2021Article
- A micromirror array with annular partitioning for high-speed random-access axial focusing.Light, science & applications · 2020Article
- Effects of excitation light polarization on fluorescence emission in two-photon light-sheet microscopy.Biomedical optics express · 2020Article
- Fast multi-directional DSLM for confocal detection without striping artifacts.Biomedical optics express · 2020Article
- Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector.Journal of biomedical optics · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of light-sheet fluorescence microscopy (LSFM) has greatly expanded the experimental capabilities in many biological and biomedical research fields, enabling for example live studies of murine and zebrafish neural activity or of cell growth and division. The key feature of the method is the selective illumination of a sample single plane, providing an intrinsic optical sectioning and allowing direct 2D image recording. On the other hand, this excitation scheme is more affected by absorption or scattering artifacts in comparison to point scanning methods, leading to un-even illumination. We present here an easily implementable method, based on acousto-optical deflectors (AOD), to overcome this obstacle. We report the advantages provided by flexible and fast AODs in generating simultaneous angled multiple beams from a single laser beam and in fast light sheet pivoting and we demonstrate the suppression of illumination artifacts.
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Registered trials
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