Evidence map›Paper›PMID 30799954›Full record

ArticleCancer management and research2019

Establishment and characterization of the GC-030-35 cell line derived from gastric hepatoid adenocarcinoma.

Jingsun Wei, Yiqi Xue, Xinying Huo, Rongbo Han, Xinyu Su, Yan Jin, Wenjing Zhao, Yuetong Chen, Honghong Zhang, Jiali Dai and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer management and research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Jingsun WeiDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, jinfeichen@njmu.edu.cn.
Yiqi XueDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, jinfeichen@njmu.edu.cn.
Xinying HuoDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, jinfeichen@njmu.edu.cn.
Rongbo HanDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, jinfeichen@njmu.edu.cn.
Xinyu SuDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, jinfeichen@njmu.edu.cn.
Yan JinDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, jinfeichen@njmu.edu.cn.
Wenjing ZhaoDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, jinfeichen@njmu.edu.cn.
Yuetong ChenDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, jinfeichen@njmu.edu.cn.
Honghong ZhangDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, jinfeichen@njmu.edu.cn.
Jiali DaiDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, jinfeichen@njmu.edu.cn.
Jinfei ChenDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, jinfeichen@njmu.edu.cn.
Nanjing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeGastric hepatoid adenocarcinoma is a rare subtype of primary gastric cancer and is a high-grade form of malignancy. However, the pathogenesis and molecular biology of gastric hepatoid adenocarcinoma remain poorly understood. The aim of this study was to establish and characterize a new human gastric hepatoid adenocarcinoma cell line, GC-030-35. MATERIALS AND

methodsThe GC-030-35 cell line was established from tumor cells from a 58-year-old Chinese man with gastric hepatoid adenocarcinoma. The cultured cells underwent immunocytochemistry and flow cytometry to confirm the tumor cell phenotype. RNA sequencing was performed to analyze the differences in gene expression between GC-030-35 cells compared with normal gastric epithelial cells. A zebrafish assay was performed. Gene enrichment analysis and interrogation of the bioinformatics databases, the Gene Ontology (GO) database and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, were used for pathway analysis.

resultsFlow cytometry analysis of the GC-030-35 cells showed a positive expression rate for CD44+ of 10.7%, high cell clonality, an average plating efficiency of 32%, cell-doubling time of 29.2 hours, and cell proliferation for >15 generations in serial culture. The zebrafish assay showed the ability of the GC-030-35 cells to proliferate, promote angiogenesis, and metastasize. RNA sequencing identified the functional clustering of 6,601 differentially expressed genes of GC-030-35, which were significantly different when compared with nonneoplastic gastric epithelial cells. Pathway enrichment analysis and interrogation of the GO and KEGG bioinformatics databases identified genes for microbial metabolism in diverse environments (63 genes), metabolism of xenobiotics by cytochrome P450 (CYP450; 25 genes), and the drug metabolism cytochrome P450 (28 genes).

conclusionA human gastric hepatoid adenocarcinoma cell line, GC-030-35, was developed and characterized by comparison with normal gastric epithelial cells. Bioinformatics and gene analysis data showed that the CYP450 gene was significantly differentially expressed by GC-030-35 cells.

Indexed as

bioinformaticscarcinogenesiscell linecytochrome P450differentially expressed genesgastric hepatoid adenocarcinoma

Identifiers

PMID30799954
PMCPMC6371931
OpenAlexW2914630607

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