Evidence map›Paper›PMID 30797224›Full record

ArticleIranian biomedical journal2019

Distribution of the CM-Dil-Labeled Human Umbilical Cord Vein Mesenchymal Stem Cells Migrated to the Cyclophosphamide-Injured Ovaries in C57BL/6 Mice

Ladan Jalalie, Mohammad Jafar Rezaie, Ali Jalili, Mohammad Ali Rezaee, Zakaria Vahabzadeh, Mohammad Reza Rahmani, Mojtaba Karimipoor, Mohammad Saeed Hakhamaneshi

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PubMed Full text at PMC
In one paragraph

Article in Iranian biomedical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 30 citations in OpenAlex.

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  10. Stem cell-based therapy for human diseases.Signal transduction and targeted therapy · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Ladan JalalieStudent Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Mohammad Jafar RezaieCellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Ali JaliliCancer and Immunology Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Mohammad Ali RezaeeZoonoses Research center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Zakaria VahabzadehLiver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Mohammad Reza RahmaniCancer and Immunology Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Mojtaba KarimipoorDepartment of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Mohammad Saeed HakhamaneshiStudent Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Kurdistan University of Medical Sciences · IRUrmia University · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Mesenchymal stem cells (MSCs) can be used to treat premature ovarian failure (POF). Different methods have already been applied to detect MSCs in tissues. This study aimed to investigate the quantitative distribution of CM-DiI-labeled human umbilical cord vein MSCs (hUCV-MSCs) in different regions of the ovarian tissue of the cyclophosphamide (CTX)-induced POF in mice. Methods: Adult female C57BL/6 mice (n = 40) were divided into four groups: (1) Mice receiving PBS as control (Ctrl) group; (2) mice receiving hUCV-MSCs intravenously as Ctrl + hUCV-MSCs group; (3) mice receiving CTX intraperitoneally (i.p.) as CTX group; (4) mice receiving CM-DiI-labeled hUCV-MSCs after CTX injection as CTX + hUCV-MSCs group. Histological changes and CM-DiI-labeled hUCV-MSCs distribution were analyzed in the ovarian tissues. Quantitative real-time PCR was performed to detect human mitochondrial cytochrome b (MTCYB) gene in the ovarian tissues of the mice. Results: The mean number of the fluorescent hUCV-MSCs was 20 ± 2.5 (57.1%) in the medulla, 11.3 ± 2.8 (32.2%) in the cortex, and 5.5 ± 1 (15%) in the germinal epithelium of the ovarian tissue (p < 0.05). Moreover, MTCYB gene was detected in the mice ovaries of the CTX + hUCV-MSCs group, but not in other groups. Conclusion: Our findings suggest that the distribution of the transplanted hUCV-MSCs in different regions of the ovarian tissue is not equal, and it is greater in the medulla than the cortex and germinal epithelium. This is the first report of quantitative distribution of MSCs in different regions of ovarian tissue in the POF model.

Indexed as

Cell MovementStaining and LabelingAnimalsCarbocyaninesCyclophosphamideCytochromes bFemaleHumansMesenchymal Stem CellsMice, Inbred C57BLOvaryPrimary Ovarian InsufficiencyUmbilical Cord3,3'-dioactadecyl-5,5'-di(4-sulfophenyl)oxacarbocyanineCarbocyaninesCyclophosphamideCytochromes bCyclophosphamideMesenchymal stem cellsPremature ovarian failureTransplantation

Identifiers

PMID30797224
PMCPMC6462299
OpenAlexW2943592090

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.