ArticleThe Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases
Effects of sub-minimum inhibitory concentrations of ciprofloxacin on biofilm formation and virulence factors of Escherichia coli.
Article in The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Beyond lethal doses: sub-MIC levels of multi-class antibiotics induce species- and isolate-dependent biofilm inhibition in gram-negative pathogens.BMC microbiology · 2026Article
- Inhibitory effects of p-coumaric acid against avian pathogenic Escherichia coli O2:K1 in vitro and in vivo.Poultry science · 2026Article
- Alteration of fimbria-mediated biofilm formation and virulence in the zoonotic pathogenMicrobiology spectrum · 2026Article
- Fosfomycin at sub-minimum inhibitory concentration impairs biofilm and efflux pump activity in multidrug-resistant Klebsiella pneumoniae isolates.BMC microbiology · 2026Article
- Isolation and identification of bacteriophage against Escherichia coli ATCC 25922 and their biofilm Inhibition studies.Scientific reports · 2025Article
- Andrographolide reverses the susceptibility of Streptococcus suis to aminoglycoside antibiotics by proton motive force.BMC veterinary research · 2025Article
- Investigation of the effect of anti-PIA/PNAG antibodies on biofilm formation inFrontiers in microbiology · 2025Article
- The SOS Response Activation and the Risk of Antibiotic Resistance Enhancement inInternational journal of molecular sciences · 2024Article
- Article
- Gram-positive bacteria are primed for surviving lethal doses of antibiotics and chemical stress.bioRxiv : the preprint server for biology · 2024Article
- Evaluation of the application potential of Bdellovibrio sp. YBD-1 isolated from Yak faeces.Scientific reports · 2024Article
- Potential antivirulence activity of sub-inhibitory concentrations of ciprofloxacin against Proteus mirabilis isolates: an in-vitro and in-vivo study.Annals of clinical microbiology and antimicrobials · 2024Article
- Effect of Sub-Inhibitory Concentrations of Nitrofurantoin, Ciprofloxacin, and Trimethoprim on In Vitro Biofilm Formation in UropathogenicMedical sciences (Basel, Switzerland) · 2022Article
- Effect of Ciprofloxacin-Loaded Niosomes onPharmaceutics · 2022Article
- Investigating the Role of Antibiotics on Induction, Inhibition and Eradication of Biofilms of Poultry AssociatedAntibiotics (Basel, Switzerland) · 2022Article
- Multifunctional Microspheres Based on D-Mannose and Resveratrol for Ciprofloxacin Release.Materials (Basel, Switzerland) · 2022Article
- Survival and Virulence Potential of Drug-ResistantInternational journal of environmental research and public health · 2022Article
- Investigation of the Role of Virulence Gene in Biofilm Formation ofArchives of Razi Institute · 2022Article
- Nanoemulsion as an Effective Inhibitor of Biofilm-forming Bacterial Associated Drug Resistance: An Insight into COVID Based Nosocomial Infections.Biotechnology and bioprocess engineering : BBE · 2022Review
- Synergy between Indoloquinolines and Ciprofloxacin: An Antibiofilm Strategy againstAntibiotics (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo evaluate the influence of sub-minimum inhibitory concentrations (MICs) of ciprofloxacin (CIP) on biofilm formation and virulence factors of Escherichia coli clinical isolates.
methodsSub-MICs of CIP were determined using growth curve experiments. The biofilm-forming capacity of E. coli clinical isolates and E. coli ATCC 25922 treated or untreated with sub-MICs of CIP was assessed using a crystal violet staining assay. The biofilm structure of E. coli isolate was assessed with scanning electron microscopy (SEM). The expression levels of the virulence genes fim, usp, and iron and the biofilm formation genes of the pgaABCD locus were measured using quantification RT-PCR (qRT-PCR) in E. coli isolates and E. coli ATCC 25922.
resultsBased on our results, the sub-MICs of CIP were 1/4 MICs. Sub-MICs of CIP significantly inhibited biofilm formation of E. coli clinical isolates and E. coli ATCC 25922 (p<0.01). SEM analyses indicated that the biofilm structure of the E. coli changed significantly after treatment with sub-MICs of CIP. Expression levels of the virulence genes fim, usp, and iron and the biofilm formation genes of the pgaABCD locus were also suppressed.
conclusionsThe results revealed that treatment with sub-MICs of CIP for 24h inhibited biofilm formation and reduced the expression of virulence genes and biofilm formation genes in E. coli.
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