ArticleMikrochimica acta2019
A fluorescent aptasensor for ochratoxin A detection based on enzymatically generated copper nanoparticles with a polythymine scaffold.
Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Aptamers used for biosensors and targeted therapy.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2020Pooled it
- Article
- Use of polypyrrole ferrite microparticles and liquid chromatography-mass spectrometry for testing natural grass contamination by multiclass mycotoxins.Mikrochimica acta · 2023Article
- In situ generated PANI promoted flexible photoelectrochemical biosensor for ochratoxin A based on GOx-stuffed DNA hydrogel as enhancer.Mikrochimica acta · 2023Article
- A Fluorescent Biosensor for Streptavidin Detection Based on Double-Hairpin DNA-Templated Copper Nanoparticles.Biosensors · 2023Article
- Development of Fluorescent Aptasensors Based on G-Quadruplex Quenching Ability for Ochratoxin A and Potassium Ions Detection.Biosensors · 2022Article
- Nanomaterial-based aptamer biosensors for ochratoxin A detection: a review.Analytical and bioanalytical chemistry · 2022Review
- Molecular Diagnostic of Ochratoxin A With Specific Aptamers in Corn and Groundnut via Fabrication of a Microfluidic Device.Frontiers in nutrition · 2022Article
- A novel aptasensor based on DNA hydrogel for sensitive visual detection of ochratoxin A.Mikrochimica acta · 2021Article
- Electrochemical impedance biosensor array based on DNAzyme-functionalized single-walled carbon nanotubes using Gaussian process regression for Cu(II) and Hg(II) determination.Mikrochimica acta · 2020Article
- Colorimetric aggregation assay based on array of gold and silver nanoparticles for simultaneous analysis of aflatoxins, ochratoxin and zearalenone by using chemometric analysis and paper based analytical devices.Mikrochimica acta · 2020Article
- A review on recent developments in optical and electrochemical aptamer-based assays for mycotoxins using advanced nanomaterials.Mikrochimica acta · 2019Review
- Nanobody-based fluorescence resonance energy transfer immunoassay for noncompetitive and simultaneous detection of ochratoxin a and ochratoxin B.Environmental pollution (Barking, Essex : 1987) · 2019Article
- Label-Free Fluorescent Aptasensor for Ochratoxin-A Detection Based on CdTe Quantum Dots and (Toxins · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A fluorescence enhancement method is presented for the determination of ochratoxin A (OTA). The interaction of OTA with its aptamer causes structural changes which, in turn, change fluorescence of enzymatically generated polythymine-coated copper nanoparticles (CuNPs) (with excitation/emission maxima at 340/625 nm). The OTA-binding aptamer was immobilized on magnetic beads. When it binds OTA, it is partially released and exposes a region with a partly complimentary DNA strand (cDNA). After magnetic separation, the cDNA was employed as a primer to trigger the terminal deoxynucleotidyl transferase-mediated polymerization. This process generates polythymine which act as a template for synthesis of the CuNPs. The method is sensitive in having a 2.0 nM detection limit for OTA. It was successfully applied to the determination of OTA in spiked diluted red wine. Graphical abstract Schematic presentation of a fluorometric enhancement method for ochratoxin A assay based on ochratoxin A inducing structure switching of its aptamer and enzymatically generated polythymine for copper nanoparticles formation.
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Registered trials
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