ArticleFunctional & integrative genomics2020
Optimized Cas9 expression systems for highly efficient Arabidopsis genome editing facilitate isolation of complex alleles in a single generation.
Article in Functional & integrative genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Specific light-harvesting complexes mediate grana stacking and prevent energy spillover between photosystems in plant chloroplasts.The Plant cell · 2026Article
- Mapping light-harvesting and photoprotection responses in the Photosystem II antenna system of higher plants.Plant physiology · 2025Article
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- The Arabidopsis PIP1;1 Aquaporin Represses Lateral Root Development and Nitrate Uptake Under Low Nitrate Availability.Plant, cell & environment · 2025Article
- Applications of CRISPR/Cas tools in improving stress tolerance inFrontiers in plant science · 2025Review
- Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency.Biotechnology journal · 2024Review
- An inducible CRISPR activation tool for accelerating plant regeneration.Plant communications · 2024Article
- Hidden prevalence of deletion-inversion bi-alleles in CRISPR-mediated deletions of tandemly arrayed genes in plants.Nature communications · 2023Article
- Article
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- An extensible vector toolkit and parts library for advanced engineering of plant genomes.The plant genome · 2023Article
- Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two.Plant methods · 2023Article
- Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding.Plants (Basel, Switzerland) · 2023Review
- Seedlessness Trait and Genome Editing-A Review.International journal of molecular sciences · 2023Review
- The heat is on: a simple method to increase genome editing efficiency in plants.BMC plant biology · 2022Article
- From Genome Sequencing to CRISPR-Based Genome Editing for Climate-Resilient Forest Trees.International journal of molecular sciences · 2022Review
- Establishment of an Efficient Genome Editing System in Lettuce Without Sacrificing Specificity.Frontiers in plant science · 2022Article
- Gene activation via Cre/lox-mediated excision in cowpea (Vigna unguiculata).Plant cell reports · 2022Article
- Increased mutation efficiency of CRISPR/Cas9 genome editing in banana by optimized construct.PeerJ · 2022Article
- Photosynthetic Light Harvesting and Thylakoid Organization in a CRISPR/Cas9 Arabidopsis Thaliana LHCB1 Knockout Mutant.Frontiers in plant science · 2022Article
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Authors and funding
7 authors.
Funding
Abstract
Genetic resources for the model plant Arabidopsis comprise mutant lines defective in almost any single gene in reference accession Columbia. However, gene redundancy and/or close linkage often render it extremely laborious or even impossible to isolate a desired line lacking a specific function or set of genes from segregating populations. Therefore, we here evaluated strategies and efficiencies for the inactivation of multiple genes by Cas9-based nucleases and multiplexing. In first attempts, we succeeded in isolating a mutant line carrying a 70 kb deletion, which occurred at a frequency of ~ 1.6% in the T
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