ArticleScientific reports2019
The crystal structure of the chitinase ChiA74 of Bacillus thuringiensis has a multidomain assembly.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 44 citations in OpenAlex.
- Computer-Based Design to Improve Bacillus thuringiensis Chitinase for Industrial Applications.ChemistryOpen · 2026Article
- Comparative Potential of Chitinase and Chitosanase from the StrainBiotech (Basel (Switzerland)) · 2025Article
- Marine chitinase AfChi: green defense management against Colletotrichum gloeosporioides and anthracnose.AMB Express · 2024Article
- Three-Step Enzymatic Remodeling of Chitin into Bioactive Chitooligomers.Journal of agricultural and food chemistry · 2024Article
- Insights into Chitin-Degradation Potential ofMicroorganisms · 2024Article
- Biodegradation and Prospect of Polysaccharide from Crustaceans.Marine drugs · 2022Review
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- Diversity of culturable bacteria endowed with antifungal metabolites biosynthetic characteristics associated with tea rhizosphere soil of Assam, India.BMC microbiology · 2021Article
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- Structural inspection and protein motions modelling of a fungal glycoside hydrolase family 18 chitinase by crystallography depicts a dynamic enzymatic mechanism.Computational and structural biotechnology journal · 2021Article
- Structural Profiling of Bacterial Effectors Reveals Enrichment of Host-Interacting Domains and Motifs.Frontiers in molecular biosciences · 2021Article
- The Crystal Structure of aInternational journal of molecular sciences · 2020Article
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- Microbial chitinases: properties, current state and biotechnological applications.World journal of microbiology & biotechnology · 2019Review
- Expression and characterization of two chitinases with synergistic effect and antifungal activity from Xenorhabdus nematophila.World journal of microbiology & biotechnology · 2019Article
- Chitinases ofFrontiers in microbiology · 2019Review
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Authors and funding
6 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
There is no structural information about any chitinase synthesized by Bacillus thuringiensis, the most successful microbial insect larvicide used worldwide. In this study, we solved the 3D structure of the chitinase ChiA74 at 2.26 Å. The crystal structure shows that ChiA74 is composed of a modular arrangement formed by (i) a catalytic region (CD), (ii) a chitinase insertion domain (CID), (iii) a fibronectin type III domain (FnIII), and (iv) a chitin binding domain (CBD). The location of the CBD with respect to the CD has no structural similarity to other chitinases with known structures. The activity of a ChiA74 lacking its secretion signal peptide (ChiA74Δsp) and a truncated version lacking its CBD/FnIII domains (ChiA74Δsp-50) did not have statistical differences in activity against colloidal chitin. However, ChiA74Δsp exhibits 4.5 and 2.0 higher activity than versions lacking the CBD (ChiA74Δsp-60) and CBD/FnIII domains (ChiA74Δsp-50), respectively, when crystalline chitin was used as substrate. Our data suggest that the CBD might plays a significant role in crystalline chitin hydrolysis. We also demonstrated the importance of the catalytic E211 in the CD, as mutants ChiA74Δsp
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