Evidence map›Paper›PMID 30796243›Full record

ArticleScientific reports2019

A method to identify respiratory virus infections in clinical samples using next-generation sequencing.

Talia Kustin, Guy Ling, Sivan Sharabi, Daniela Ram, Nehemya Friedman, Neta Zuckerman, Efrat Dahan Bucris, Aharona Glatman-Freedman, Adi Stern, Michal Mandelboim

Abstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  5. The respiratory tract virome: unravelling the role of viral dark matter in respiratory health and disease.European respiratory review : an official journal of the European Respiratory Society · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Talia KustinSchool of Molecular Cell Biology and Biotechnology, George S. Wise Faculty of Life Sciences Tel Aviv University, Tel Aviv, Israel.
Guy LingSchool of Molecular Cell Biology and Biotechnology, George S. Wise Faculty of Life Sciences Tel Aviv University, Tel Aviv, Israel.
Sivan SharabiCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, Ramat-Gan, Israel.
Daniela RamCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, Ramat-Gan, Israel.
Nehemya FriedmanCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, Ramat-Gan, Israel.
Neta ZuckermanCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, Ramat-Gan, Israel.
Efrat Dahan BucrisCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, Ramat-Gan, Israel.
Aharona Glatman-FreedmanDepartment of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Adi SternSchool of Molecular Cell Biology and Biotechnology, George S. Wise Faculty of Life Sciences Tel Aviv University, Tel Aviv, Israel. sternadi@post.tau.ac.il.
Michal MandelboimCentral Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Tel-Hashomer, Ramat-Gan, Israel. michalman@sheba.health.gov.il.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Respiratory virus infections are very common. Such infections impose an enormous economic burden and occasionally lead to death. Furthermore, every few decades, respiratory virus pandemics emerge, putting the entire world population at risk. Thus, there is an urgent need to quickly and precisely identify the infecting agent in a clinical setting. However, in many patients with influenza-like symptoms (ILS) the identity of the underlying pathogen remains unknown. In addition, it takes time and effort to individually identify the virus responsible for the ILS. Here, we present a new next-generation sequencing (NGS)-based method that enables rapid and robust identification of pathogens in a pool of clinical samples without the need for specific primers. The method is aimed at rapidly uncovering a potentially common pathogen affecting many samples with an unidentified source of disease.

Indexed as

BacteriaHigh-Throughput Nucleotide SequencingHumansInfluenza, HumanReproducibility of ResultsRespiratory Tract InfectionsSeasonsVirus DiseasesViruses

Identifiers

PMID30796243
PMCPMC6384955

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.