Evidence map›Paper›PMID 30794719›Full record

ArticleGenetics and molecular biology

Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae).

Juliana Rodrigues Bombonato, Isabel Aparecida Silva Bonatelli, Gislaine Angélica Rodrigues Silva, Evandro Marsola Moraes, Daniela Cristina Zappi, Nigel P Taylor, Fernando Faria Franco

Open access · goldAbstract read
In one paragraph

Article in Genetics and molecular biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Juliana Rodrigues BombonatoDepartamento de Biologia, Centro de Ciências Humanas e Biológicas, Universidade Federal de São Carlos, Sorocaba, SP, Brazil.ORCID http://orcid.org/0000-0002-4479-9454
Isabel Aparecida Silva BonatelliDepartamento de Biologia, Centro de Ciências Humanas e Biológicas, Universidade Federal de São Carlos, Sorocaba, SP, Brazil.ORCID http://orcid.org/0000-0002-0930-1411
Gislaine Angélica Rodrigues SilvaDepartamento de Biologia, Centro de Ciências Humanas e Biológicas, Universidade Federal de São Carlos, Sorocaba, SP, Brazil.ORCID http://orcid.org/0000-0003-4222-5593
Evandro Marsola MoraesDepartamento de Biologia, Centro de Ciências Humanas e Biológicas, Universidade Federal de São Carlos, Sorocaba, SP, Brazil.ORCID http://orcid.org/0000-0003-4197-0794
Daniela Cristina ZappiInstituto Tecnológico Vale, Museu Paraense Emilio Goeldi, Coord. Botânica, Belém, PA, Brazil.ORCID http://orcid.org/0000-0001-6755-2238
Nigel P TaylorSingapore Botanic Gardens (National Parks Board), Singapore, Republic of Singapore.
Fernando Faria FrancoDepartamento de Biologia, Centro de Ciências Humanas e Biológicas, Universidade Federal de São Carlos, Sorocaba, SP, Brazil.ORCID http://orcid.org/0000-0001-9597-5713
Universidade Federal de São Carlos · BRMuseu Paraense Emílio Goeldi · BRNational Parks Board · SG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus.

Identifiers

PMID30794719
PMCPMC6428128
OpenAlexW2917795825

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.