ArticlePloS one2019
Optimum temperature may be a misleading parameter in enzyme characterization and application.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- The Prominent Role of Serines 302/307 in the Activity and Stability of Human Caspase9: Appraisal of the S302D and S307D Variants.Biochemical genetics · 2026Article
- Machine learning for enzyme catalytic activity: current progress and future horizons.Briefings in bioinformatics · 2026Review
- Seq2Topt: a sequence-based deep learning predictor of enzyme optimal temperature.Briefings in bioinformatics · 2025Article
- Homogenization-circulating ultrasound in combination with aqueous enzymatic pretreatment for microwave-assisted extraction of kernel oil and essential oil from the fruit of Litsea cubeba.Ultrasonics sonochemistry · 2024Article
- Influence of deep eutectic solvents on redox biocatalysis involving alcohol dehydrogenases.Heliyon · 2024Review
- Treatment of Pelvic and Spinal Bone Metastases: Radiotherapy and Hyperthermia Alone vs. in Combination.Cancers · 2024Article
- Data-driven strategies for the computational design of enzyme thermal stability: trends, perspectives, and prospects.Acta biochimica et biophysica Sinica · 2023Review
- Article
- Purification, identification and characterization of Nag2 N-acetylglucosaminidase from Trichoderma virens strain mango.Botanical studies · 2022Article
- Thermally stable and acidic pH tolerant mutant phytases with high catalytic efficiency from Yersinia intermedia for potential application in feed industries.Environmental science and pollution research international · 2022Article
- Review
- Article
- Extraction and Purification of Extracellular L-Glutamate Oxidase fromArchives of Razi Institute · 2021Article
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2 authors.
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Abstract
The optimum temperature is commonly determined in enzyme characterization. A search in the PubMed database for "optimum temperature" and "enzymes" yielded more than 1,700 manuscripts reporting this parameter over the last five years. Here, we show that the optimum temperature is not a constant. The catalytic activity of the mesophylic β-glucosidase Sfβgly was determined at different temperatures using different assay times and enzyme concentrations. We observed that the optimum temperature for Sfβgly changed by 5°C simply by modifying the assay length, and it was inversely correlated with enzyme concentration. These observations rely on the fact that close to the melting temperature, thermal denaturation continuously decreases the active enzyme concentration as the assay progresses. Thus, as the denaturation rate increases with increasing temperature, the bell-shaped curves observed in "activity versus temperature plots" occur only if the enzyme is denatured at and above the optimum temperature, which was confirmed using the thermostable β-glucosidase bglTm. Thus, the optimum temperature hardly reflects an intrinsic enzyme property and is actually a mere consequence of the assay condition. Thus, adoption of the "optimum temperature" determined under bench conditions for large-scale uses, which differ in assay length and enzyme concentration, may result in lower yields and financial losses.
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