Evidence map›Paper›PMID 30794676›Full record

ArticlePloS one2019

Antibiotic treatment-induced dysbiosis differently affects BDNF and TrkB expression in the brain and in the gut of juvenile mice.

Michela Bistoletti, Valentina Caputi, Nicolò Baranzini, Nicoletta Marchesi, Viviana Filpa, Ilaria Marsilio, Silvia Cerantola, Genciana Terova, Andreina Baj, Annalisa Grimaldi and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed, 2 pooled it
10.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 2 syntheses or guidelines pooled it, 101 citations in OpenAlex.

  1. Pooled it
  2. The microbiota-gut- hippocampus axis.Frontiers in neuroscience · 2022
    Pooled it
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  14. BDNF/TrkB activators in Parkinson's disease: A new therapeutic strategy.Journal of cellular and molecular medicine · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 2 countries.

Michela BistolettiDepartment of Medicine and Surgery, University of Insubria, Varese, Italy.
Valentina CaputiDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
Nicolò BaranziniDepartment of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
Nicoletta MarchesiDepartment of Drug Science, University of Pavia, Pavia, Italy.
Viviana FilpaDepartment of Medicine and Surgery, University of Insubria, Varese, Italy.
Ilaria MarsilioDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
Silvia CerantolaDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
Genciana TerovaDepartment of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
Andreina BajDepartment of Medicine and Surgery, University of Insubria, Varese, Italy.
Annalisa GrimaldiDepartment of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
Alessia PascaleDepartment of Drug Science, University of Pavia, Pavia, Italy.
Gianmario FrigoDepartment of Internal Medicine and Therapeutics, Section of Pharmacology, University of Pavia, Pavia, Italy.
Francesca CremaDepartment of Internal Medicine and Therapeutics, Section of Pharmacology, University of Pavia, Pavia, Italy.
Maria Cecilia GironDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
Cristina GiaroniDepartment of Medicine and Surgery, University of Insubria, Varese, Italy.ORCID 0000-0002-3760-3441
University of Insubria · ITUniversity of Padua · ITUniversity of Pavia · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antibiotic use during adolescence may result in dysbiosis-induced neuronal vulnerability both in the enteric nervous system (ENS) and central nervous system (CNS) contributing to the onset of chronic gastrointestinal disorders, such as irritable bowel syndrome (IBS), showing significant psychiatric comorbidity. Intestinal microbiota alterations during adolescence influence the expression of molecular factors involved in neuronal development in both the ENS and CNS. In this study, we have evaluated the expression of brain-derived neurotrophic factor (BDNF) and its high-affinity receptor tropomyosin-related kinase B (TrkB) in juvenile mice ENS and CNS, after a 2-week antibiotic (ABX) treatment. In both mucosa and mucosa-deprived whole-wall small intestine segments of ABX-treated animals, BDNF and TrKB mRNA and protein levels significantly increased. In longitudinal muscle-myenteric plexus preparations of ABX-treated mice the percentage of myenteric neurons staining for BDNF and TrkB was significantly higher than in controls. After ABX treatment, a consistent population of BDNF- and TrkB-immunoreactive neurons costained with SP and CGRP, suggesting up-regulation of BDNF signaling in both motor and sensory myenteric neurons. BDNF and TrkB protein levels were downregulated in the hippocampus and remained unchanged in the prefrontal cortex of ABX-treated animals. Immunostaining for BDNF and TrkB decreased in the hippocampus CA3 and dentate gyrus subregions, respectively, and remained unchanged in the prefrontal cortex. These data suggest that dysbiosis differentially influences the expression of BDNF-TrkB in the juvenile mice ENS and CNS. Such changes may potentially contribute later to the development of functional gut disorders, such as IBS, showing psychiatric comorbidity.

Indexed as

AnimalsAnti-Bacterial AgentsBrainBrain-Derived Neurotrophic FactorDysbiosisEnteric Nervous SystemGene Expression RegulationIrritable Bowel SyndromeMembrane GlycoproteinsMiceNeuronsProtein-Tyrosine KinasesReceptor, trkBSignal TransductionAnti-Bacterial AgentsBdnf protein, mouseBrain-Derived Neurotrophic FactorMembrane GlycoproteinsNtrk2 protein, mouseProtein-Tyrosine KinasesReceptor, trkB

Identifiers

PMID30794676
PMCPMC6386304
OpenAlexW2917593984

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.