ArticleProtoplasma2019
Two grapevine metacaspase genes mediate ETI-like cell death in grapevine defence against infection of Plasmopara viticola.
Article in Protoplasma, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- The role of plant metacaspases in cell death and survival.Cell death and differentiation · 2026Review
- Unleash the dogs of death: metacaspase 5, microtubules, and hypersensitive response.Plant cell reports · 2025Article
- Advances in the molecular mechanism of grapevine resistance to fungal diseases.Molecular horticulture · 2025Review
- Kinetin induces microtubular breakdown, cell cycle arrest and programmed cell death in tobacco BY-2 cells.Protoplasma · 2023Article
- Genome editing for healthy crops: traits, tools and impacts.Frontiers in plant science · 2023Review
- Regulating Death and Disease: Exploring the Roles of Metacaspases in Plants and Fungi.International journal of molecular sciences · 2022Review
- Jasmonic acid contributes to rice resistance against Magnaporthe oryzae.BMC plant biology · 2022Article
- The grapevine aspartic protease gene family: characterization and expression modulation in response to Plasmopara viticola.Journal of plant research · 2022Article
- Evolutionary Diversity and Function of Metacaspases in Plants: Similar to but Not Caspases.International journal of molecular sciences · 2022Review
- Transcriptional Profiling of Resistant and Susceptible Cultivars of Grapevine (Frontiers in microbiology · 2022Article
- Pyramiding of scald resistance genes in four spring barley MAGIC populations.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Review
- Defense and Offense Strategies: The Role of Aspartic Proteases in Plant-Pathogen Interactions.Biology · 2021Review
- Biotechnological Approaches: Gene Overexpression, Gene Silencing, and Genome Editing to Control Fungal and Oomycete Diseases in Grapevine.International journal of molecular sciences · 2020Review
- The Effect of Transcription FactorInternational journal of molecular sciences · 2020Article
- Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules.Frontiers in plant science · 2020Article
- Transcriptomic analysis of Chinese wild Vitis pseudoreticulata in response to Plasmopara viticola.Protoplasma · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metacaspase, as hypersensitive response (HR) executors, has been identified in many plant species. Previously, the entire gene family of metacaspase has been uncovered, but there are still questions that remain unclear regarding HR-regulating gene members. In this study, based on metacaspase expression during different grapevine genotypes interacting with Plasmopara viticola, we identified MC2 and MC5 as candidates involved in HR. We overexpressed both metacaspases as GFP fusions in tobacco BY-2 cells to address subcellular localization and cellular functions. We found MC2 located at the ER, while MC5 was nucleocytoplasmic. In these overexpressor lines, cell death elicited by the bacterial protein harpin, is significantly enhanced, indicating MC2 and MC5 mediated defence-related programmed cell death (PCD). This effect was mitigated, when the membrane-located NADPH oxidase was inhibited by the specific inhibitor diphenylene iodonium, or when cells were complemented with methyl jasmonate, a crucial signal of basal immunity. Both findings are consistent with a role of MC2 and MC5 in cell death-related immunity. Using a dual-luciferase reporter system in grapevine cells we demonstrated both MC2 and MC5 promoter alleles from V. rupestris were more responsive to harpin than those from V. vinifera cv 'Müller-Thurgau', while they were not induced by MeJA as signal linked with basal immunity. These findings support a model, where MC2 and MC5 act specifically as executors of the HR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.