Evidence map›Paper›PMID 30792979›Full record

ArticleFrontiers in chemistry2019

Free Polyethylenimine Enhances Substrate-Mediated Gene Delivery on Titanium Substrates Modified With RGD-Functionalized Poly(acrylic acid) Brushes.

Amy Mantz, Alice Rosenthal, Eric Farris, Tyler Kozisek, Eva Bittrich, Saghar Nazari, Eva Schubert, Mathias Schubert, Manfred Stamm, Petra Uhlmann and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 3 countries.

Amy MantzDepartment of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE, United States.
Alice RosenthalLeibniz-Institut für Polymerforschung Dresden e.V., Dresden, Germany.
Eric FarrisDepartment of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE, United States.
Tyler KozisekDepartment of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE, United States.
Eva BittrichLeibniz-Institut für Polymerforschung Dresden e.V., Dresden, Germany.
Saghar NazariLeibniz-Institut für Polymerforschung Dresden e.V., Dresden, Germany.
Eva SchubertCenter for Nanohybrid Functional Materials, University of Nebraska-Lincoln, Lincoln, NE, United States.
Mathias SchubertCenter for Nanohybrid Functional Materials, University of Nebraska-Lincoln, Lincoln, NE, United States.
Manfred StammLeibniz-Institut für Polymerforschung Dresden e.V., Dresden, Germany.
Petra UhlmannLeibniz-Institut für Polymerforschung Dresden e.V., Dresden, Germany.
Angela K PannierDepartment of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE, United States.
University of Nebraska–Lincoln · USLeibniz Institute of Polymer Research · DETU Dresden · DE

Funding

Using Cell Priming and Telecommunications Modeling to Enhance Gene Delivery for Stem Cell TherapiesDP2EB025760 · NIBIB · UNIVERSITY OF NEBRASKA LINCOLN · PI PANNIER, ANGELA K · 2017 to 2022
$2.8M
NIBIB NIH HHS DP2 EB025760
6 · The paper itself

Abstract

Substrate mediated gene delivery (SMD) is a method of immobilizing DNA complexes to a substrate via covalent attachment or nonspecific adsorption, which allows for increased transgene expression with less DNA compared to traditional bolus delivery. It may also increase cells receptivity to transfection via cell-material interactions. Substrate modifications with poly(acrylic) acid (PAA) brushes may improve SMD by enhancing substrate interactions with DNA complexes via tailored surface chemistry and increasing cellular adhesion via moieties covalently bound to the brushes. Previously, we described a simple method to graft PAA brushes to Ti and further demonstrated conjugation of cell adhesion peptides (i.e., RGD) to the PAA brushes to improve biocompatibility. The objective of this work was to investigate the ability of Ti substrates modified with PAA-RGD brushes (PAA-RGD) to immobilize complexes composed of branched polyethyleneimine and DNA plasmids (bPEI-DNA) and support SMD in NIH/3T3 fibroblasts. Transfection in NIH/3T3 cells cultured on bPEI-DNA complexes immobilized onto PAA-RGD substrates was measured and compared to transfection in cells cultured on control surfaces with immobilized complexes including Flat Ti, PAA brushes modified with a control peptide (RGE), and unmodified PAA. Transfection was two-fold higher in cells cultured on PAA-RGD compared to those cultured on all control substrates. While DNA immobilization measured with radiolabeled DNA indicated that all substrates (PAA-RGD, unmodified PAA, Flat Ti) contained nearly equivalent amounts of loaded DNA, ellipsometric measurements showed that more total mass (i.e., DNA and bPEI, both complexed and free) was immobilized to PAA and PAA-RGD compared to Flat Ti. The increase in adsorbed mass may be attributed to free bPEI, which has been shown to improve transfection. Further transfection investigations showed that removing free bPEI from the immobilized complexes decreased SMD transfection and negated any differences in transfection success between cells cultured on PAA-RGD and on control substrates, suggesting that free bPEI may be beneficial for SMD in cells cultured on bPEI-DNA complexes immobilized on PAA-RGD grafted to Ti. This work demonstrates that substrate modification with PAA-RGD is a feasible method to enhance SMD outcomes on Ti and may be used for future applications such as tissue engineering, gene therapy, and diagnostics.

Indexed as

branched polyethyleniminenonviral gene deliverypoly(acrylic) acidpolymer brushesRGD ligandsubstrate mediated

Identifiers

PMID30792979
PMCPMC6374293
OpenAlexW2912145981

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.