ArticleFrontiers in chemistry2019
Development of Structure-Switching Aptamers for Kanamycin Detection Based on Fluorescence Resonance Energy Transfer.
Article in Frontiers in chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 27 citations in OpenAlex.
- Recent Advances of Fluorescent Aptasensors for the Detection of Antibiotics in Food.Biosensors · 2025Review
- Enhancing Target Detection: A Fluorescence-Based Streptavidin-Bead Displacement Assay.Biosensors · 2024Article
- A universal optical aptasensor for antibiotics determination based on a new high-efficiency Förster resonance energy transfer pair.Mikrochimica acta · 2024Article
- Rational Approach to Optimizing Conformation-Switching Aptamers for Biosensing Applications.ACS sensors · 2024Article
- A massively parallel screening platform for converting aptamers into molecular switches.Nature communications · 2023Article
- Chlortetracycline-Functionalized Silver Nanoparticles as a Colorimetric Probe for Aminoglycosides: Ultrasensitive Determination of Kanamycin and Streptomycin.Nanomaterials (Basel, Switzerland) · 2020Article
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6 authors at 1 institution in 1 country.
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Abstract
The structure-switching aptamers are designed for the simple and rapid detection of kanamycin based on the signal transduction principle of fluorescence resonance energy transfer (FRET). The structure switch is composed of kanamycin-binding aptamers and the complementary strands, respectively labeled with fluorophore and quencher, denoted as FDNA and QDNA. In the absence of kanamycin, FDNA and QDNA form the double helix structure through the complementary pairing of bases. The fluorophore and the quencher are brought into close proximity, which results in the fluorescence quenching because of the FRET mechanism. In the presence of kanamycin, the FDNA specifically bind to the target due to the high affinity of aptamers, and the QDNA are dissociated. The specific recognition between aptamers and kanamycin will obstruct the formation of structure switch and reduce the efficiency of FRET between FDNA and QDNA, thus leading to the fluorescence enhancement. Therefore, based on the structure-switching aptamers, a simple fluorescent assay for rapid detection of kanamycin was developed. Under optimal conditions, there was a good linear relationship between kanamycin concentration and the fluorescence signal recovery. The linear range of this method in milk samples was 100-600 nM with the detection limit of 13.52 nM (
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