ArticleScientific reports2019
Polyacrylamide injection matrix for serial femtosecond crystallography.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
- A simple and practical approach for protein serial crystallography using grease matrix and large-area support film.Journal of synchrotron radiation · 2026Article
- Serial femtosecond crystallography data processing at the global science data hub center at KISTI.Scientific reports · 2026Article
- Standard Sample Preparation for Serial Femtosecond Crystallography.Biomolecules · 2025Review
- Effect of crystal-to-detector distance shift on data processing in serial crystallography.PloS one · 2025Article
- Data of fixed-target pink-beam serial synchrotron crystallography at the Pohang Light Source II.Data in brief · 2024Article
- Combination of an inject-and-transfer system for serial femtosecond crystallography.Journal of applied crystallography · 2022Article
- Beef tallow injection matrix for serial crystallography.Scientific reports · 2022Article
- Crystal structure of CmABCB1 multi-drug exporter in lipidic mesophase revealed by LCP-SFX.IUCrJ · 2022Article
- Article
- Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography.Scientific reports · 2021Article
- Room-Temperature Structure of Xylitol-Bound Glucose Isomerase by Serial Crystallography: Xylitol Binding in the M1 Site Induces Release of Metal Bound in the M2 Site.International journal of molecular sciences · 2021Article
- Lard Injection Matrix for Serial Crystallography.International journal of molecular sciences · 2020Article
- Polysaccharide-Based Injection Matrix for Serial Crystallography.International journal of molecular sciences · 2020Article
- Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography.Journal of applied crystallography · 2020Article
- Viscosity-adjustable grease matrices for serial nanocrystallography.Scientific reports · 2020Article
- Shortening injection matrix for serial crystallography.Scientific reports · 2020Article
- Nylon mesh-based sample holder for fixed-target serial femtosecond crystallography.Scientific reports · 2019Article
- Sample Delivery Media for Serial Crystallography.International journal of molecular sciences · 2019Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serial femtosecond crystallography (SFX) provides opportunities to observe the dynamics of macromolecules without causing radiation damage at room temperature. Although SFX provides a biologically more reliable crystal structure than provided by the existing synchrotron sources, there are limitations due to the consumption of many crystal samples. A viscous medium as a carrier matrix reduces the flow rate of the crystal sample from the injector, thereby dramatically reducing sample consumption. However, the currently available media cannot be applied to specific crystal samples owing to reactions between the viscous medium and crystal sample. The discovery and characterisation of a new delivery medium for SFX can further expand its use. Herein, we report the preparation of a polyacrylamide (PAM) injection matrix to determine the crystal structure with an X-ray free-electron laser. We obtained 11,936 and 22,213 indexed images using 0.5 mg lysozyme and 1.0 mg thermolysin, respectively. We determined the crystal structures of lysozyme and thermolysin delivered in PAM at 1.7 Å and 1.8 Å resolutions. The maximum background scattering from PAM was lower than monoolein, a commonly used viscous medium. Our results show that PAM can be used as a sample delivery media in SFX studies.
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