ArticleScientific reports2019
A framework for tracer-based metabolism in mammalian cells by NMR.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Article
- Live Metabolomics with NMR.Analytical chemistry · 2026Review
- Probing Intracellular Yeast Metabolism With Deuterium Magnetic Resonance Spectroscopy.NMR in biomedicine · 2025Article
- Methods and Guidelines for Metabolism Studies: Applications to Cancer Research.International journal of molecular sciences · 2025Review
- Metabolomics and Proteomics in Prostate Cancer Research: Overview, Analytical Techniques, Data Analysis, and Recent Clinical Applications.International journal of molecular sciences · 2024Review
- Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Low-field NMR with multilayer Halbach magnet and NMR selective excitation.Scientific reports · 2023Article
- Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Metabolomics in Cell Biology.Handbook of experimental pharmacology · 2023Article
- Metabolomics of infectious diseases in the era of personalized medicine.Frontiers in molecular biosciences · 2023Review
- Practical Aspects of NMR-Based Metabolomics.Handbook of experimental pharmacology · 2023Article
- NMR-Based Metabolomics to Evaluate Individual Response to Treatments.Handbook of experimental pharmacology · 2023Article
- Isolated murine skeletal muscles utilize pyruvate over glucose for oxidation.Metabolomics : Official journal of the Metabolomic Society · 2022Article
- Article
- Untargeted 2D NMR Metabolomics of [Journal of proteome research · 2022Article
- Multidimensional engineered metasurface for ultrafast terahertz switching at frequency-agile channels.Nanophotonics (Berlin, Germany) · 2022Article
- Studying Metabolism by NMR-Based Metabolomics.Frontiers in molecular biosciences · 2022Review
- Targeting Asparagine and Serine Metabolism in Germinal Centre-Derived B Cells Non-Hodgkin Lymphomas (B-NHL).Cells · 2021Article
- Article
- Ketohexokinase inhibition improves NASH by reducing fructose-induced steatosis and fibrogenesis.JHEP reports : innovation in hepatology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Metabolism changes extensively during the normal proliferation and differentiation of mammalian cells, and in cancer and inflammatory diseases. Since changes in the metabolic network reflect interactions between genetic, epigenetic and environmental changes, it is helpful to study the flow of label from isotopically labelled precursors into other metabolites rather than static metabolite levels. For this Nuclear Magnetic Resonance (NMR) spectroscopy is an attractive technique as it can quantify site-specific label incorporation. However, for applications using human cells and cell lines, the challenge is to optimize the process to maximize sensitivity and reproducibility. Here we present a new framework to analyze metabolism in mammalian cell lines and primary cells, covering the workflow from the preparation of cells to the acquisition and analysis of NMR spectra. We have applied this new approach in hematological and liver cancer cell lines and confirm the feasibility of tracer-based metabolism in primary liver cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.