Evidence map›Paper›PMID 30792265›Full record

ArticleMolecular & cellular proteomics : MCP2019

Streamlined Protocol for Deep Proteomic Profiling of FAC-sorted Cells and Its Application to Freshly Isolated Murine Immune Cells.

Samuel A Myers, Andrew Rhoads, Alexandra R Cocco, Ryan Peckner, Adam L Haber, Lawrence D Schweitzer, Karsten Krug, D R Mani, Karl R Clauser, Orit Rozenblatt-Rosen and 3 more

Abstract read
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.

0numbers the graph read from it
0cells of the map it votes in
71citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

71 citing papers in PubMed.

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11 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Samuel A MyersFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142;. Electronic address: sammyers@broadinstitute.org.
Andrew Rhoads¶Division of Immunology, Department of Microbiology and Immunobiology, Harvard Medical School, and Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital, Boston, Massachusetts.
Alexandra R CoccoFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142.
Ryan PecknerFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142.
Adam L HaberFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
Lawrence D SchweitzerFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142.
Karsten KrugFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142.
D R ManiFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142.
Karl R ClauserFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142.
Orit Rozenblatt-RosenFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
Nir HacohenFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;; ‖Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02215;; **Center for Cancer Immunology, Massachusetts General Hospital, Boston, Massachusetts 02114.
Aviv RegevFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142;; ‡‡Howard Hughes Medical Institute and Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02140.
Steven A CarrFrom the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;. Electronic address: scarr@broad.mit.edu.

Funding

Pilot & Feasibility ProgramP30DK043351 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI RAYMOND T CHUNG · 1991 to 2026
$35.3M
Center for Cell CircuitsRM1HG006193 · NHGRI · BROAD INSTITUTE, INC. · PI BLAINEY, PAUL CLARK, HACOHEN, NIR · 2016 to 2020
$14.9M
MICROSCALED PROTEOGENOMICS FOR CANCER CLINICAL TRIALSU01CA214125 · NCI · BAYLOR COLLEGE OF MEDICINE · PI ANURAG, MEENAKSHI, CARR, STEVEN A · 2017 to 2021
$7.0M
Center of Excellence for High Throughput Proteogenomic CharacterizationU24CA210986 · NCI · BROAD INSTITUTE, INC. · PI CARR, STEVEN A, GILLETTE, MICHAEL A · 2016 to 2020
$6.4M
Integrated high throughput proteogenomic data analysis center for CPTACU24CA210979 · NCI · BROAD INSTITUTE, INC. · PI BIRGER, CHET, GETZ, GAD A · 2016 to 2020
$4.4M
NCI NIH HHS U01 CA214125NCI NIH HHS U24 CA210979NCI NIH HHS U24 CA210986NHGRI NIH HHS RM1 HG006193NIDDK NIH HHS P30 DK043351
6 · The paper itself

Abstract

Proteomic profiling describes the molecular landscape of proteins in cells immediately available to sense, transduce, and enact the appropriate responses to extracellular queues. Transcriptional profiling has proven invaluable to our understanding of cellular responses; however, insights may be lost as mounting evidence suggests transcript levels only moderately correlate with protein levels in steady state cells. Mass spectrometry-based quantitative proteomics is a well-suited and widely used analytical tool for studying global protein abundances. Typical proteomic workflows are often limited by the amount of sample input that is required for deep and quantitative proteome profiling. This is especially true if the cells of interest need to be purified by fluorescence-activated cell sorting (FACS) and one wants to avoid

Indexed as

Cell SeparationFlow CytometryAnimalsImmune SystemLeukocytesMaleMice, Inbred C57BLPeptidesProteomeProteomicsRNATranscription, GeneticPeptidesProteomeRNAAnimal models*Cell sortingFACSGene Expression*Immunology*Low InputPost-transcriptional regulationSystems biology*TMT

Identifiers

PMID30792265
PMCPMC6495249

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.