Evidence map›Paper›PMID 30791357›Full record

ReviewInternational journal of molecular sciences2019

Applications of the CRISPR/Cas9 System for Rice Grain Quality Improvement: Perspectives and Opportunities.

Sajid Fiaz, Shakeel Ahmad, Mehmood Ali Noor, Xiukang Wang, Afifa Younas, Aamir Riaz, Adeel Riaz, Fahad Ali

Abstract readReview
In one paragraph

Review in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Review
  8. Review
  9. Advances in Rice Seed Shattering.International journal of molecular sciences · 2023
    Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Mutation of barleyFrontiers in plant science · 2022
    Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sajid FiazState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. sajidfiaz50@yahoo.com.ORCID 0000-0001-9097-4359
Shakeel AhmadState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. shakeelpbg@gmail.com.ORCID 0000-0001-6651-9399
Mehmood Ali NoorInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, Beijing 100081, China. mehmood2017@gmail.com.ORCID 0000-0003-2459-2357
Xiukang WangCollege of Life Sciences, Yan'an University, Yan'an 716000, Shaanxi, China. wangxiukang@126.com.ORCID 0000-0001-5638-809X
Afifa YounasDepartment of Botany, Lahore College for Women University, Lahore 54000, Pakistan. afifa_younas@hotmail.com.
Aamir RiazState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. aamirriaz33@gmail.com.
Adeel RiazBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. adeelpopy@yahoo.com.ORCID 0000-0002-8957-4099
Fahad AliState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China. fahadali.pbg@hotmail.com.

Funding

National Natural Science Foundation of China 51669034, 41761068, 51809224
6 · The paper itself

Abstract

Grain quality improvement is a key target for rice breeders, along with yield. It is a multigenic trait that is simultaneously influenced by many factors. Over the past few decades, breeding for semi-dwarf cultivars and hybrids has significantly contributed to the attainment of high yield demands but reduced grain quality, which thus needs the attention of researchers. The availability of rice genome sequences has facilitated gene discovery, targeted mutagenesis, and revealed functional aspects of rice grain quality attributes. Some success has been achieved through the application of molecular markers to understand the genetic mechanisms for better rice grain quality; however, researchers have opted for novel strategies. Genomic alteration employing genome editing technologies (GETs) like clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) for reverse genetics has opened new avenues of research in the life sciences, including for rice grain quality improvement. Currently, CRISPR/Cas9 technology is widely used by researchers for genome editing to achieve the desired biological objectives, because of its simple targeting. Over the past few years many genes that are related to various aspects of rice grain quality have been successfully edited via CRISPR/Cas9 technology. Interestingly, studies on functional genomics at larger scales have become possible because of the availability of GETs. In this review, we discuss the progress made in rice by employing the CRISPR/Cas9 editing system and its eminent applications. We also elaborate possible future avenues of research with this system, and our understanding regarding the biological mechanism of rice grain quality improvement.

Indexed as

CRISPR-Cas SystemsGene EditingEdible GrainFood QualityGenome, PlantGenomicsMutagenesisNutritive ValueOryzaQuality ImprovementStarchStarchCRISPR/Cas9functional genomicsOryza sativa L. molecular markersreverse genetics

Identifiers

PMID30791357
PMCPMC6412304

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.