ArticlePloS one2019
A new approach for Small Ruminant Lentivirus full genome characterization revealed the circulation of divergent strains.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Article
- Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25.The veterinary quarterly · 2025Article
- Investigation of an association betweenJournal of veterinary research · 2025Article
- Permissiveness of different TMEM154 genotype cell lines to different SRLV genotypes/subtypes.Journal of virology · 2025Article
- Genetic landscape of Italian SRLV: insights from passive surveillance-based phylogenetic analysis.Frontiers in microbiology · 2025Article
- First Report of SNPs Detection in TMEM154 Gene in Sheep from Poland and Their Association with SRLV Infection Status.Pathogens (Basel, Switzerland) · 2024Article
- Molecular Characterization of Small Ruminant Lentiviruses in Sheep and Goats: A Systematic Review.Animals : an open access journal from MDPI · 2024Review
- Identification of New Single Nucleotide Polymorphisms Potentially Related to Small Ruminant Lentivirus Infection Susceptibility in Goats Based on Data Selected from High-Throughput Sequencing.Pathogens (Basel, Switzerland) · 2024Article
- Detection of small ruminant Lentivirus proviral DNA in red deer from Poland.BMC veterinary research · 2024Article
- A Combined Approach for the Characterization of Small Ruminant Lentivirus Strains Circulating in the Islands and Mainland of Greece.Animals : an open access journal from MDPI · 2024Article
- Serological and Molecular Characterization of Small Ruminant Lentiviruses in Morocco.Animals : an open access journal from MDPI · 2024Article
- Distribution of maedi-visna virus in different organs of the spontaneously affected small ruminants in India.Iranian journal of veterinary research · 2024Article
- The genetic variability of small-ruminant lentiviruses and its impact on tropism, the development of diagnostic tests and vaccines and the effectiveness of control programmes.Journal of veterinary research · 2023Article
- First Molecular Characterization of Small Ruminant Lentiviruses Detected in Romania.Animals : an open access journal from MDPI · 2023Article
- Longitudinal Study on Seroreactivity of Goats Exposed to Colostrum and Milk of Small Ruminant Lentivirus-infected Dams.Journal of veterinary research · 2022Article
- Serological Survey of Small Ruminant Lentivirus Infections in Free-Ranging Mouflon and Chamois in Slovenia.Animals : an open access journal from MDPI · 2022Article
- Molecular Characterization of Small Ruminant Lentiviruses Isolated from Polish Goats with Arthritis.Viruses · 2022Article
- Evaluation of Serological Methods and a New Real-Time Nested PCR for Small Ruminant Lentiviruses.Pathogens (Basel, Switzerland) · 2022Article
- Article
- Quasispecies Composition of Small Ruminant Lentiviruses Found in Blood Leukocytes and Milk Epithelial Cells.Viruses · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small Ruminant Lentiviruses (SRLV) include at least 4 viral highly divergent genotypes. Genotypes A and B are widely distributed and genotypes C and E have been recognized in restricted geographic areas. New phylogroups have been identified targeting conserved regions. However, this approach suffers from the potential risk to misamplify highly divergent strains. Pathogenic strains are easily adapted to fibroblastic cells, but non-pathogenic strains isolation may require a different approach. We developed a fast and effective method for SRLV full genome characterization after cell culture isolation. Spleen samples were collected during regular slaughter from sheep and goats in northwestern Italy. Spleen-derived macrophage cultures were monitored for reverse transcriptase activity and RNA was extracted from the supernatant of positive cultures. Using Illumina MiSeq platform 22 new full genome sequences were obtained. The success of this approach is based on the following features: spleen is one of the main target for SRLV persistence; red pulp is a reserve of resident macrophages, the main target for SRLV replication in vivo; RTA is a sensitive assay for any replicating retrovirus; de novo sequencing do not require genetic knowledge in advance.
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