ArticleParasitology research2019
Prevalence and molecular characterisation of Sarcocystis miescheriana and Sarcocystis suihominis in wild boars (Sus scrofa) in Italy.
Article in Parasitology research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Global Prevalence and Future Prediction of Sarcocystis Infection in Pigs: A Systematic Review and Meta-Analysis.Acta parasitologica · 2026Pooled it
- Molecular Occurrence, Co-infections and Genetic Characterization of Sarcocystis miescheriana, Toxoplasma gondii and Neospora caninum in Wild Boars (Sus scrofa) from Türkiye.Acta parasitologica · 2026Article
- Article
- Rapid suspension-based screening ofInternational journal for parasitology. Parasites and wildlife · 2026Article
- Monitoring the health of wolves (Canis lupus): Integrating conservation and public health.PloS one · 2026Article
- High frequency of DNA detection ofFood and waterborne parasitology · 2025Article
- Article
- Morphological and molecular characterisation of Sarcocystis capracanis, Sarcocystis cornagliai and Sarcocystis rossii n. sp. infecting the Alpine ibex (Capra ibex).Parasites & vectors · 2025Article
- Infection survey, molecular, pathogenicity, and morphological characteristics of Sarcocystis species naturally infected water buffaloes (Bubalus bubalis) in Egypt.BMC veterinary research · 2024Article
- First molecular characterisation ofJournal of veterinary research · 2024Article
- Gross lesions associated with Sarcocystis miescheriana in a wild boar hunted for human consumption: the importance of trained hunters to ensure animal health surveillance and food safety.Parasitology research · 2024Article
- Microscopic detection and molecular characterization of Sarcocystis miescheriana in wild boars (Sus scrofa): first report from Greece.Parasitology research · 2024Article
- Molecular identification ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Sero-epidemiological investigation on Toxoplasma gondii infection in Apennine wolf (Canis lupus italicus) and wild boar (Sus scrofa) in Italy.BMC veterinary research · 2024Article
- Red foxes (Frontiers in veterinary science · 2024Article
- Molecular identification of Sarcocystis species in sika deer (Cervus nippon) of free-ranging populations in Germany and Austria.Veterinary research communications · 2023Article
- Macroscopic sarcocystosis in a pig carcass from an Italian abattoir.Veterinary research communications · 2023Article
- Serological Prevalence ofAnimals : an open access journal from MDPI · 2023Article
- First report ofInternational journal for parasitology. Parasites and wildlife · 2023Article
- Prevalence ofAnimals : an open access journal from MDPI · 2023Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A sample of the diaphragm was collected from each of 100 wild boars legally hunted in the Val Grande National Park in north-western Italy and examined for the presence of Sarcocystis infection by histological and molecular methods. In histological sections, thick-walled sarcocysts consistent with those of Sarcocystis miescheriana were detected in 32 wild boars. Genomic DNA extracted from diaphragm samples was initially subjected to PCR amplification of the internal transcribed spacer 1 (ITS1) region, and 97 wild boars were found to harbour a Sarcocystis infection at this screening. Selected DNA samples were then subjected to PCR amplification and sequencing of the ITS1 region and the 18S and 28S ribosomal RNA (rRNA) genes of the nuclear ribosomal DNA unit, while all positive samples were subjected to PCR amplification of the mitochondrial cytochrome c oxidase subunit I (cox1) gene. S. miescheriana was identified in 97 wild boars (97%), while the zoonotic Sarcocystis suihominis was identified in one wild boar (1%), which also harboured S. miescheriana. Intra-specific sequence variation was found in all four DNA regions of S. miescheriana examined and in the 18S rRNA gene and ITS1 region of S. suihominis. The partial cox1 gene was amplified and sequenced from 72 isolates of S. miescheriana, yielding 43 haplotypes with pairwise sequence identities of 97.6-99.9%. These haplotypes were 79.1-79.8% identical with the cox1 sequence of S. suihominis. Phylogeny based on cox1 sequences placed S. miescheriana and S. suihominis as sister species within a clade comprising mainly Sarcocystis spp. of ruminants with felids as known or presumed definitive hosts. The same was true for the phylogeny based on 18S rRNA gene sequences.
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