ArticleNature communications2019
Cytosolic lipid droplets as engineered organelles for production and accumulation of terpenoid biomaterials in leaves.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 70 citations in OpenAlex.
- Metabolic engineering for tobacco quality improvement: Advances and perspectives.Metabolic engineering communications · 2026Review
- Protein engineering strategies to optimise recombinant product synthesis and accumulation in Nicotiana benthamiana.Plant biotechnology journal · 2026Review
- A multifunctional sesquiterpene synthase integrates with cytochrome P450s to reinforce the terpenoid defense network in maize.The Plant journal : for cell and molecular biology · 2025Article
- The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets.The Plant cell · 2025Article
- Enhancing terpenoid production in Saccharomyces cerevisiae via cell morphology engineering.Biotechnology letters · 2025Article
- Towards rational control of seed oil composition: dissecting cellular organization and flux control of lipid metabolism.Plant physiology · 2025Review
- Chemical characterization, pathway enrichments and bioactive potentials of catechin-producing endophytic fungi isolated from tea leaves.RSC advances · 2024Article
- Exploring selection signatures in the divergence and evolution of lipid droplet (LD) associated genes in major oilseed crops.BMC genomics · 2024Article
- Plant Engineering to Enable Platforms for Sustainable Bioproduction of Terpenoids.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Compartmentalized Terpenoid Production in Plants Using Agrobacterium-Mediated Transient Expression.Methods in molecular biology (Clifton, N.J.) · 2024Article
- EngineeringmLife · 2023Article
- Improved antioxidant activities of spice require enrichment of distinct yet closely-related metabolic pathways.Heliyon · 2023Article
- Maize Terpene Synthase 8 (ZmTPS8) Contributes to a Complex Blend of Fungal-Elicited Antibiotics.Plants (Basel, Switzerland) · 2023Article
- Engineering a universal and efficient platform for terpenoid synthesis in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Comparative transcriptome and metabolome analyses of fourJournal of ginseng research · 2023Article
- Pathway Engineering, Re-targeting, and Synthetic Scaffolding Improve the Production of Squalene in Plants.ACS synthetic biology · 2022Article
- A glossary of plant cell structures: Current insights and future questions.The Plant cell · 2022Review
- Another level of complex-ity: The role of metabolic channeling and metabolons in plant terpenoid metabolism.Frontiers in plant science · 2022Review
- Transporter Engineering in Microbial Cell Factory Boosts Biomanufacturing Capacity.Biodesign research · 2022Review
- Isolation of Lipid Droplets for Protein and Lipid Analysis.Methods in molecular biology (Clifton, N.J.) · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosolic lipid droplets are endoplasmic reticulum-derived organelles typically found in seeds as reservoirs for physiological energy and carbon to fuel germination. Here, we report synthetic biology approaches to co-produce high-value sesqui- or diterpenoids together with lipid droplets in plant leaves. The formation of cytosolic lipid droplets is enhanced in the transient Nicotiana benthamiana system through ectopic production of WRINKLED1, a key regulator of plastid fatty acid biosynthesis, and a microalgal lipid droplet surface protein. Engineering of the pathways providing the universal C5-building blocks for terpenoids and installation of terpenoid biosynthetic pathways through direction of the enzymes to native and non-native compartments boost the production of target terpenoids. We show that anchoring of distinct biosynthetic steps onto the surface of lipid droplets leads to efficient production of terpenoid scaffolds and functionalized terpenoids. The co-produced lipid droplets "trap" the terpenoids in the cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.