ArticleJournal of orthopaedic surgery and research2019
Bovine bone particulates containing bone anabolic factors as a potential xenogenic bone graft substitute.
Article in Journal of orthopaedic surgery and research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- A Pilot Investigation Generating an Alternative Therapeutic Strategy Combining Deproteinized Bovine Bone and Gelatin Sponge for a Novel Implant Material in Sinus Floor Elevation Operation.Medical science monitor : international medical journal of experimental and clinical research · 2021Trial
- Predictability, Skeletal Stability, and Safety of Iliac Crest Bone Grafts in Large Maxillary Advancement with Le Fort I Osteotomy: A Systematic Review.Journal of clinical medicine · 2026Review
- Effects of polycaprolactone/hydroxyapatite nanocomposite scaffolds combined with platelet-rich fibrin for repair of rabbit calvarial bone defects.Veterinary research forum : an international quarterly journal · 2026Article
- Bone Regeneration in Defects Created on Rat Calvaria Grafted with Porcine Xenograft and Synthetic Hydroxyapatite Reinforced with Titanium Particles-A Microscopic and Histological Study.Journal of functional biomaterials · 2025Article
- Cytotoxicity of tranexamic acid to tendon and bone in vitro: Is there a safe dosage?Journal of orthopaedic surgery and research · 2022Article
- Periodontal ligament stem cell-based bioactive constructs for bone tissue engineering.Frontiers in bioengineering and biotechnology · 2022Review
- Sustained Delivery of Lactoferrin Using Poloxamer Gels for Local Bone Regeneration in a Rat Calvarial Defect Model.Materials (Basel, Switzerland) · 2021Article
- Comparative Evaluation of Biocompatibility Data from the Subcutaneous and the Calvaria Implantation Model of a Novel Bone Substitute Block.In vivo (Athens, Greece)Article
- Evaluation of the effect of moringa oleifera gel and autologo platelet-rich fibrin in the treatment of rabbit intra bony defects. (Radio graphic and Histological study).Journal of oral and maxillofacial pathology : JOMFPArticle
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Authors and funding
12 authors.
Funding
Abstract
backgroundAlternative grafts are needed to improve the healing of bone non-union. Here, we assessed a bovine bone product which retains the inorganic and organic components of bone, as an alternative bone graft.
methodsBovine bone matrix proteins (BBMPs) were isolated from bovine bone particulates (BBPs) and tested in vitro. Primary rat osteoblast viability, differentiation, and mineralisation were assessed with alamarBlue®, real-time PCR, and von Kossa staining assays, respectively. Osteoclast formation was assessed in primary murine bone marrow cultures with TRAP staining. Human osteoblast growth and differentiation in the presence of BBPs was evaluated in 3D collagen gels in vitro using alamarBlue® and real-time PCR, respectively. The efficacy of BBPs as an alternative bone graft was tested in a rat critical-size calvarial defect model, with histology scored at 4 and 12 weeks post-surgery.
resultsIn vitro, the highest concentration of BBMPs increased mineral deposition five-fold compared to the untreated control group (P < 0.05); enhanced the expression of key osteoblast genes encoding for RUNX2, alkaline phosphatase, and osteocalcin (P < 0.05); and decreased osteoclast formation three-fold, compared to the untreated control group (P < 0.05). However, the BBPs had no effect on primary human osteoblasts in vitro, and in vivo, no difference was found in healing between the BBP-treated group and the untreated control group.
conclusionsOverall, despite the positive effects of the BBMPs on the cells of the bone, the bovine bone product as a whole did not enhance bone healing. Finding a way to harness the positive effect of these BBMPs would provide a clear benefit for healing bone non-union.
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