ReviewAnnual review of biophysics2019
Split Green Fluorescent Proteins: Scope, Limitations, and Outlook.
Review in Annual review of biophysics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
127 citing papers in PubMed.
- Advanced imaging of membrane contact sites.Nature cell biology · 2026Review
- Synaptic Ca2+ channels and neurexins are linked through direct and indirect binding complexes.Scientific reports · 2026Article
- A conserved Arf-GEF modulates axonal integrity through RAB-35 by altering neuron-epidermal attachment.Journal of cell science · 2026Article
- Protein-protein interaction detection in plants: from classical approaches to future perspectives.Biologia futura · 2026Review
- Condensate corona-nanoparticle complexes transfer functional biomolecules between cells.Nature materials · 2026Article
- The fluorescence-activating and absorption-shifting tag (FAST), a versatile protein marker for live plant cell imaging.The Plant journal : for cell and molecular biology · 2026Article
- A thyroid hormone-mediated opsin switch initiates metamorphosis in a proto-vertebrate.Science advances · 2026Article
- Article
- Magneto-Photonic Gene Circuit for Minimally Invasive Control of Gene Expression in Mammalian Cells.ACS omega · 2026Article
- CF2H: a cell-free two-hybrid platform for rapid protein binder screening.Nature communications · 2026Article
- Targeting RNA binding proteins with small-molecule inhibitors: Advances, challenges, and therapeutic opportunities.Acta pharmaceutica Sinica. B · 2026Review
- Pathogenic Gαo Mutants Drive Dominant GPCR Coupling in GNAO1 Encephalopathies.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Article
- FRET-FLIM: Unveiling Transcription Factor Interactions in Plants.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Targeted protein degradation: species, diseases and efficient utilization.Journal of translational medicine · 2025Review
- Article
- A guide to characterizing the dynamic mitochondria-endoplasmic reticulum contact sites.The FEBS journal · 2025Review
- Evolution of split-protein technologies in virology: From mechanistic discovery and diagnostics to therapeutic promise.Journal of virus eradication · 2025Review
- Magneto-Photonic Gene Circuit for Minimally Invasive Control of Gene Expression in Mammalian Cells.bioRxiv : the preprint server for biology · 2025Article
- Engineering a new tripartite split-ccGFP system from Corynactis californica for detecting protein-protein interactions.Scientific reports · 2025Article
67 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein-protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.