ArticleMolecular biology of the cell2019
The putative G protein-coupled receptor GrlD mediates extracellular polyphosphate sensing in Dictyostelium discoideum.
Article in Molecular biology of the cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Phosphoproteomic Profiling Reveals Overlapping and Distinct Signaling Pathways inbioRxiv : the preprint server for biology · 2025Article
- Two endogenousProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Starvation Induces Extracellular Accumulation of Polyphosphate inInternational journal of molecular sciences · 2023Article
- Dictyostelium discoideum cells retain nutrients when the cells are about to outgrow their food source.Journal of cell science · 2022Article
- Phylogeny-wide analysis of G-protein coupled receptors in social amoebas and implications for the evolution of multicellularity.Open research Europe · 2022Article
- Inorganic Polyphosphate: Coacervate Formation and Functional Significance in Nanomedical Applications.International journal of nanomedicine · 2022Review
- Article
- Model systems for studying polyphosphate biology: a focus on microorganisms.Current genetics · 2021Review
- Polyphosphate is an extracellular signal that can facilitate bacterial survival in eukaryotic cells.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Inorganic polyphosphate in platelet rich plasma accelerates re-epithelializationRegenerative therapy · 2020Article
- Platelet polyphosphate induces fibroblast chemotaxis and myofibroblast differentiation.Journal of thrombosis and haemostasis : JTH · 2020Article
- An improved shotgun antisense method for mutagenesis and gene identification.BioTechniques · 2020Article
- Extracellular signaling in Dictyostelium.The International journal of developmental biology · 2019Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Five or more orthophosphates bound together by high-energy phosphoanhydride bonds are highly ubiquitous inorganic molecules called polyphosphate. Polyphosphate acts as a signaling molecule eliciting a number of responses in eukaryotic cells, but the mechanisms mediating these effects are poorly understood. Proliferating Dictyostelium discoideum cells accumulate extracellular polyphosphate. At extracellular concentrations similar to those observed in stationary phase cells, polyphosphate inhibits proteasome activity and proliferation, and induces aggregation. Here we identify GrlD as a putative G protein-coupled receptor that mediates binding of extracellular polyphosphate to the cell surface. Cells lacking GrlD do not respond to polyphosphate-induced proteasome inhibition, aggregation, or proliferation inhibition. Polyphosphate also elicits differential effects on cell-substratum adhesion and cytoskeletal F-actin levels based on nutrient availability, and these effects were also mediated by GrlD. Starving cells also accumulate extracellular polyphosphate. Starved cells treated with exopolyphosphatase failed to aggregate effectively, suggesting that polyphosphate also acts as a signaling molecule during starvation-induced development of Dictyostelium. Together, these results suggest that a eukaryotic cell uses a G protein-coupled receptor to mediate the sensing and response to extracellular polyphosphate.
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