Evidence map›Paper›PMID 30785507›Full record

ArticleActa cirurgica brasileira2019

The effect of hirudin on antagonisting thrombin induced apoptosis of human microvascular endothelial cells1.

Jiangying Zhu, Xinyuan Pan, Bojie Lin, Guanyu Lin, Rohan Pradhan, Feiwen Long, Guoqian Yin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta cirurgica brasileira, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jiangying ZhuMD, Department of Plastic and Aesthetic Surgery, The First Affiliated Hospital of Guangxi Medical University, China. Conception and design of the study, manuscript preparation, final approval.ORCID http://orcid.org/0000-0002-6147-6286
Xinyuan PanMD, Department of Plastic and Aesthetic Surgery, The First Affiliated Hospital of Guangxi Medical University, China. Acquisition, analysis and interpretation of data.
Bojie LinMD, Department of Plastic and Aesthetic Surgery, The First Affiliated Hospital of Guangxi Medical University, China. Acquisition, analysis and interpretation of data.
Guanyu LinIMM, Department of Plastic and Aesthetic Surgery, The First Affiliated Hospital of Guangxi Medical University, China. Technical procedures.
Rohan PradhanIMM, Department of Plastic and Aesthetic Surgery, The First Affiliated Hospital of Guangxi Medical University, China. Technical procedures.
Feiwen LongMM, Department of Ultrasonic, Maternal and Child Health Hospital of Guangxi Zhuang Autonous Region, China. Acquisition of data.
Guoqian YinMD, Department of Plastic and Aesthetic Surgery, The First Affiliated Hospital of Guangxi Medical University, China. Analysis and interpretation of data, critical revision.
Guangxi Medical University · CNGuangxi Maternal and Child Health Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo investigate whether hirudin exerts its antithrombin action to decrease the ratio of Human Microvascular Endothelial Cells (HMVECs) apoptosis.

methodsHuman microvascular endothelial cells (HMVECs) cultured in the third and fifth generations were used. HMVECs were divided into normal group, thrombin group (T group), natrual hirudin group (H group), thrombin + natrual hirudin group (T + H group), AG490 group, thrombin + AG490 group (T + AG490 group), natrual hirudin + AG490 group (H + AG490 group), thrombin + natural hirudin + AG490 (T + H + AG490 group).Apart from the normal group, the other groups were exposed to the relevant drugs for 24 hours.HMVEC apoptosis was assessed by flow cytometric and double Immunofluorescence of phosphorylation of JAK (P-JAK2) and TUNEL assay.

resultsCompared with the normal group, in thrombin group the HMVECs apoptosis rate were significantly increased (P<0.05).The results indicated that the index of apoptosis and the apoptosis rate were improved in cultures treated by natural hirudin (T + H group), relative to cultures with thrombin only (T group). We found that the index of apoptosis and the apoptosis rate in the AG490 + thrombin group were higher than that in the hirudin + thrombin group (P<0.05). Double Immunofluorescence of p-JAK2 and TUNEL assays showed that cells were double positive for P-JAK2 uptake and TUNEL detection liquid binding.

conclusionThe natural hirudin and JAK2/STATs signal inhibitor AG490 could block the effects of thrombin. Natural hirudin could attenuate HMVECs apoptosis via antagonizing thrombin and it is suggested that this effect may occur by blocking the JAK2/STATs signaling pathway and this signaling pathways appears to be not the only pathway.

Indexed as

AntithrombinsApoptosisCell ProliferationEndothelial CellsHirudinsHumansMicrovesselsProtein Kinase InhibitorsSignal TransductionThrombinAntithrombinsHirudinsProtein Kinase InhibitorsThrombin

Identifiers

PMID30785507
PMCPMC6585924
OpenAlexW2912944774

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.