Evidence map›Paper›PMID 30785097›Full record

ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2019

Growth phase-specific changes in the composition of E. coli transcription complexes.

Isaac R Eason, Harman P Kaur, Katherine A Alexander, Maxim V Sukhodolets

Open access · greenAbstract read
In one paragraph

Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Interactions and Insertion ofInternational journal of molecular sciences · 2023
    Article
  3. Article
  4. Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq.International journal of molecular sciences · 2022
    Article
  5. The bacterial protein Hfq: Stable modifications and growth phase-dependent changes in SPAM profiles.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2021
    Article
  6. Article
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Isaac R EasonDepartment of Chemistry and Biochemistry, Lamar University, Beaumont, TX 77710, United States of America.
Harman P KaurDepartment of Chemistry and Biochemistry, Lamar University, Beaumont, TX 77710, United States of America.
Katherine A AlexanderDepartment of Chemistry and Biochemistry, Lamar University, Beaumont, TX 77710, United States of America.
Maxim V SukhodoletsDepartment of Chemistry and Biochemistry, Lamar University, Beaumont, TX 77710, United States of America. Electronic address: msoukhodol@lamar.edu.
Lamar University · US

Funding

Analyses of growth phase-specific changes in the protein composition and small protein-associated molecule makeup of E. coli transcription complexesR15GM116094 · NIGMS · LAMAR UNIVERSITY · PI SUKHODOLETS, MAXIM V · 2015 to 2015
$304k
NIGMS NIH HHS R15 GM116094
6 · The paper itself

Abstract

In E. coli, a single oligomeric enzyme transcribes the genomic DNA, while multiple auxiliary proteins and regulatory RNA interact with the core RNA polymerase (RP) during different stages of the transcription cycle to influence its function. In this work, using fast protein isolation techniques combined with mass spectrometry (MS) and immuno-analyses, we studied growth phase-specific changes in the composition of E. coli transcription complexes. We show that RP isolated from actively growing cells is represented by prevalent double copy assemblies and single copy RP-RNA and RP-RNA-RapA complexes. We demonstrate that RpoD/σ

Indexed as

Escherichia coliChromatography, High Pressure LiquidDNA-Directed RNA PolymerasesElectrophoresis, Polyacrylamide GelEscherichia coli ProteinsMacromolecular SubstancesRNA, BacterialTranscription, GeneticDNA-Directed RNA PolymerasesEscherichia coli ProteinsMacromolecular SubstancesRNA, BacterialGroELHldDRapARNA polymeraseRpoARpoDSecBTranscriptionTranslation

Identifiers

PMID30785097
PMCPMC7418856
OpenAlexW2913689823

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.