ArticleNucleic acids research2019
Structural basis of dimerization and dual W-box DNA recognition by rice WRKY domain.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 89 citations in OpenAlex.
- Functional and regulatory complexity: challenges and prospects for understanding rice WRKY transcription factors.Journal of experimental botany · 2026Review
- Genome-wide identification of the WRKY gene family in apricot (Prunus armeniaca L.) and functional characterization of PaWRKY57 in salt stress tolerance.BMC plant biology · 2026Article
- The SnRK1-RAP2.4h-PIP2 module contributes to the trade-off between growth and hypoxia tolerance in plants.Nature communications · 2026Article
- Natural Variation in TaFAD8-D Promoter Enhances Thermotolerance in Wheat Through Fatty Acid and Lipid Remodelling.Plant biotechnology journal · 2026Article
- Structural basis for sequence-specific DNA recognition by a group IId WRKY transcription factor GhWRKY17 in cotton.The Biochemical journal · 2026Article
- Unravelling the role of WRKY transcription factors in leaf senescence: Genetic and molecular insights.Journal of advanced research · 2025Review
- The transcription factor WRKY25 can act as redox switch to drive the expression of WRKY53 during leaf senescence in arabidopsis.Scientific reports · 2025Article
- PhWRKY30 activates salicylic acid biosynthesis to positively regulate antiviral defense response in petunia.Horticulture research · 2025Article
- Analysis of WRKY Gene Family inGenes · 2025Article
- Article
- RiceSNP-BST: a deep learning framework for predicting biotic stress-associated SNPs in rice.Briefings in bioinformatics · 2024Article
- A comprehensive analysis of the WRKY family in soybean and functional analysis of GmWRKY164-GmGSL7c in resistance to soybean mosaic virus.BMC genomics · 2024Article
- In vivo transgenic studies confirm the critical acylation function of LeBAHD56 for shikonin in Lithospermum erythrorhizon.Plant cell reports · 2024Article
- Structural basis for the regulation of plant transcription factor WRKY33 by the VQ protein SIB1.Communications biology · 2024Article
- Genome-wide identification and expression analysis of the WRKY gene family inFrontiers in plant science · 2024Article
- Walnut phosphatase 2A proteins interact with basic leucine zipper protein JrVIP1 to regulate osmotic stress responseForestry research · 2024Article
- Sea-ATI unravels novel vocabularies of plant active cistrome.Nucleic acids research · 2023Article
- Article
- Review
- Genome-Wide Analysis of WRKY Transcription Factors Involved in Abiotic Stress and ABA Response inInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In rice, the critical regulator of the salicylic acid signalling pathway is OsWRKY45, a transcription factor (TF) of the WRKY TF family that functions by binding to the W-box of gene promoters, but the structural basis of OsWRKY45/W-box DNA recognition is unknown. Here, we show the crystal structure of the DNA binding domain of OsWRKY45 (OsWRKY45-DBD, i.e. the WRKY and zinc finger domain) in complex with a W-box DNA. Surprisingly, two OsWRKY45-DBD molecules exchange β4-β5 strands to form a dimer. The domain swapping occurs at the hinge region between the β3 and β4 strands, and is bridged and stabilized by zinc ion via coordinating residues from different chains. The dimer contains two identical DNA binding domains that interact with the major groove of W-box DNA. In addition to hydrophobic and direct hydrogen bonds, water mediated hydrogen bonds are also involved in base-specific interaction between protein and DNA. Finally, we discussed the cause and consequence of domain swapping of OsWRKY45-DBD, and based on our work and that of previous studies present a detailed mechanism of W-box recognition by WRKY TFs. This work reveals a novel dimerization and DNA-binding mode of WRKY TFs, and an intricate picture of the WRKY/W-box DNA recognition.
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