ArticleScientific reports2019
High-throughput, Efficient, and Unbiased Capture of Small RNAs from Low-input Samples for Sequencing.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Extracellular Vesicles in Cardiovascular Disease: Intercellular Signaling, Liquid Biopsy Biomarkers, and Therapeutic Translation.Circulation research · 2026Review
- Neuropathic Pain: Mapping the miRNA Landscape.Non-coding RNA · 2026Review
- An absolute quantification atlas of small non-coding RNAs across diverse mammalian tissues and cell lines.Nature communications · 2026Article
- Preparation and processing of dried blood spots for microRNA sequencing.Biology methods & protocols · 2023Review
- G1/S restriction point coordinates phasic gene expression and cell differentiation.Nature communications · 2022Article
- Influence of feeder cells on transcriptomic analysis of pluripotent stem cells.Cell proliferation · 2022Article
- Global MicroRNA Profiling of Vascular Endothelial Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Mechanosensitive miR-100 coordinates TGFβ and Wnt signaling in osteocytes during fluid shear stress.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Single-cell microRNA sequencing method comparison and application to cell lines and circulating lung tumor cells.Nature communications · 2021Article
- Small RNA-Sequencing: Approaches and Considerations for miRNA Analysis.Diagnostics (Basel, Switzerland) · 2021Review
- A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol.Nucleic acids research · 2021Article
- A digital PCR system based on the thermal cycled chip with multi helix winding capillary.Scientific reports · 2020Article
- Comparative transcriptome analysis of root, stem, and leaf tissues of Entada phaseoloides reveals potential genes involved in triterpenoid saponin biosynthesis.BMC genomics · 2020Article
- Optimization of small RNA library preparation protocol from human urinary exosomes.Journal of translational medicine · 2020Article
- Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays.Microsystems & nanoengineering · 2020Article
- Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
MicroRNAs hold great promise as biomarkers of disease. However, there are few efficient and robust methods for measuring microRNAs from low input samples. Here, we develop a high-throughput sequencing protocol that efficiently captures small RNAs while minimizing inherent biases associated with library production. The protocol is based on early barcoding such that all downstream manipulations can be performed on a pool of many samples thereby reducing reagent usage and workload. We show that the optimization of adapter concentrations along with the addition of nucleotide modifications and random nucleotides increases the efficiency of small RNA capture. We further show, using unique molecular identifiers, that stochastic capture of low input RNA rather than PCR amplification influences the biased quantitation of intermediately and lowly expressed microRNAs. Our improved method allows the processing of tens to hundreds of samples simultaneously while retaining high efficiency quantitation of microRNAs in low input samples from tissues or bodily fluids.
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