ArticleScientific reports2019
Transcriptome-derived investigation of biosynthesis of quinolizidine alkaloids in narrow-leafed lupin (Lupinus angustifolius L.) highlights candidate genes linked to iucundus locus.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Proteomics and Alkaloid Signature of Narrow Leaf Lupin Leaves Reveal Key Enzymes in Quinolizidine Alkaloid Biosynthesis and Transportation.Journal of agricultural and food chemistry · 2026Article
- Identification of a new QTL associated to reduced quinolizidine alkaloid content in white lupin (Lupinus albus, L.) and development of ultra-low alkaloid recombinants by stacking with the pauper allele.BMC plant biology · 2025Article
- Article
- Genomic structure of yellow lupin (Lupinus luteus): genome organization, evolution, gene family expansion, metabolites and protein synthesis.BMC genomics · 2025Article
- Development and validation of PCR marker array for molecular selection towards spring, vernalization-independent and winter, vernalization-responsive ecotypes of white lupin (Lupinus albus L.).Scientific reports · 2025Article
- The causal mutation leading to sweetness in modern white lupin cultivars.Science advances · 2023Article
- Gain or Loss? Evidence for Legume Predisposition to Symbiotic Interactions with Rhizobia via Loss of Pathogen-Resistance-Related Gene Families.International journal of molecular sciences · 2022Article
- A pan-genome and chromosome-length reference genome of narrow-leafed lupin (Lupinus angustifolius) reveals genomic diversity and insights into key industry and biological traits.The Plant journal : for cell and molecular biology · 2022Article
- Integrative analysis of the steroidal alkaloids distribution and biosynthesis of bulbs Fritillariae Cirrhosae through metabolome and transcriptome analyses.BMC genomics · 2022Article
- Genetic manipulation of anti-nutritional factors in major crops for a sustainable diet in future.Frontiers in plant science · 2022Review
- Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin.Plant methods · 2021Article
- Integrated transcriptome and small RNA sequencing analyses reveal a drought stress response network in Sophora tonkinensis.BMC plant biology · 2021Article
- Pangenome of white lupin provides insights into the diversity of the species.Plant biotechnology journal · 2021Article
- Expression Profiles of Alkaloid-Related Genes across the Organs of Narrow-Leafed Lupin (International journal of molecular sciences · 2021Article
- A Bitter-Sweet Story: Unraveling the Genes Involved in Quinolizidine Alkaloid Synthesis inFrontiers in plant science · 2021Review
- Analysis of Genetic Diversity in the Traditional Chinese Medicine Plant 'Kushen' (Frontiers in plant science · 2021Article
- Phenotypic characterisation and linkage mapping of domestication syndrome traits in yellow lupin (Lupinus luteus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- The Effects of Domestication on Secondary Metabolite Composition in Legumes.Frontiers in genetics · 2020Review
- Chromatographic Fingerprinting of the Old World Lupins Seed Alkaloids: A Supplemental Tool in Species Discrimination.Plants (Basel, Switzerland) · 2019Article
- Candidate Domestication-Related Genes Revealed by Expression Quantitative Trait Loci Mapping of Narrow-Leafed Lupin (International journal of molecular sciences · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unravelling the biosynthetic pathway of quinolizidine alkaloids (QAs), regarded as antinutritional compounds of narrow-leafed lupin (NLL) seeds, is fundamental to best exploit NLL as food or feed. We investigated 12 candidate genes connected to QA biosynthesis, selecting them by transcriptomic and genomic approaches, from the landscape of genes differentially expressed in leaves of the high- and low-alkaloid NLL accessions. Linkage analysis enabled the assessment of the location of the candidate genes in relation to iucundus, a major locus of unknown identity, that confers reduced QA content in seeds. The key finding was the identification of APETALA2/ethylene response transcription factor, RAP2-7, cosegregating with the iucundus locus and located within a region with highly significant QTLs that affect QA composition. We additionally identified a 4-hydroxy-tetrahydrodipicolinate synthase (DHDPS) gene involved in L-lysine biosynthesis as being closely linked to iucundus. The distributed location of other remaining candidates (including previously known QA genes) across different linkage groups, also indirectly supports the transcription factor as a possible regulator of lupin alkaloid biosynthesis. Our findings provide crucial insight into QA biosynthesis in NLL. Additionally, we evaluated and selected appropriate reference genes for qRT-PCRs to analyse the expression levels of QA genes in NLL.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.