ArticleProceedings of the National Academy of Sciences of the United States of America2019
Beating the reaction limits of biosensor sensitivity with dynamic tracking of single binding events.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Dynamic Single-Binding Event Profiling With on-Chip Microlenses for Wash-Free Digital Biosensing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A label-free optical biosensor-based point-of-care test for the rapid detection of Monkeypox virus.Biosensors & bioelectronics · 2025Article
- A Label-free Optical Biosensor-Based Point-of-Care Test for the Rapid Detection of Monkeypox Virus.medRxiv : the preprint server for health sciences · 2024Article
- A high-dimensional microfluidic approach for selection of aptamers with programmable binding affinities.Nature chemistry · 2023Article
- A high-throughput single-particle imaging platform for antibody characterization and a novel competition assay for therapeutic antibodies.Scientific reports · 2023Article
- Label-free single-particle imaging approach for ultra-rapid detection of pathogenic bacteria in clinical samples.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Attomolar sensitivity microRNA detection using real-time digital microarrays.Scientific reports · 2022Article
- Boosting the detection performance of severe acute respiratory syndrome coronavirus 2 test through a sensitive optical biosensor with new superior antibody.Bioengineering & translational medicine · 2022Article
- Label-free optical biosensors in the pandemic era.Nanophotonics (Berlin, Germany) · 2022Review
- Determination of the Highly Sensitive Carboxyl-Graphene Oxide-Based Planar Optical Waveguide Localized Surface Plasmon Resonance Biosensor.Nanomaterials (Basel, Switzerland) · 2022Article
- The quantum dotChemical science · 2022Article
- Dynamic single-molecule sensing by actively tuning binding kinetics for ultrasensitive biomarker detection.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Microscopies Enabled by Photonic Metamaterials.Sensors (Basel, Switzerland) · 2022Review
- Evaluation of Polytyramine Film and 6-Mercaptohexanol Self-Assembled Monolayers as the Immobilization Layers for a Capacitive DNA Sensor Chip: A Comparison.Sensors (Basel, Switzerland) · 2021Article
- Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins.Chemical reviews · 2021Review
- Vibrational Spectroscopic Detection of a Single Virus by Mid-Infrared Photothermal Microscopy.Analytical chemistry · 2021Article
- Gradient-Based Rapid Digital Immunoassay for High-Sensitivity Cardiac Troponin T (hs-cTnT) Detection in 1 μL Plasma.ACS sensors · 2021Article
- Enhancement of COVID-19 detection time by means of electrothermal force.Microfluidics and nanofluidics · 2021Article
- A computational simulation platform for designing real-time monitoring systems with application to COVID-19.Biosensors & bioelectronics · 2021Article
- Biosensors-on-Chip: An Up-to-Date Review.Molecules (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The clinical need for ultrasensitive molecular analysis has motivated the development of several endpoint-assay technologies capable of single-molecule readout. These endpoint assays are now primarily limited by the affinity and specificity of the molecular-recognition agents for the analyte of interest. In contrast, a kinetic assay with single-molecule readout could distinguish between low-abundance, high-affinity (specific analyte) and high-abundance, low-affinity (nonspecific background) binding by measuring the duration of individual binding events at equilibrium. Here, we describe such a kinetic assay, in which individual binding events are detected and monitored during sample incubation. This method uses plasmonic gold nanorods and interferometric reflectance imaging to detect thousands of individual binding events across a multiplex solid-phase sensor with a large area approaching that of leading bead-based endpoint-assay technologies. A dynamic tracking procedure is used to measure the duration of each event. From this, the total rates of binding and debinding as well as the distribution of binding-event durations are determined. We observe a limit of detection of 19 fM for a proof-of-concept synthetic DNA analyte in a 12-plex assay format.
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