Evidence map›Paper›PMID 30782654›Full record

ArticlemBio2019

Structure and Mechanism of LcpA, a Phosphotransferase That Mediates Glycosylation of a Gram-Positive Bacterial Cell Wall-Anchored Protein.

Sara D Siegel, Brendan R Amer, Chenggang Wu, Michael R Sawaya, Jason E Gosschalk, Robert T Clubb, Hung Ton-That

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in mBio, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
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  8. Effect of NaCl stress on exoproteome profiles ofFrontiers in microbiology · 2023
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  11. Topical application ofInternational journal of molecular medicine · 2022
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  12. Genetic and molecular determinants of polymicrobial interactions inProceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  13. The dental plaque biofilm matrix.Periodontology 2000 · 2021
    Review
  14. Review
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  17. Phenotypic Switching ofFrontiers in microbiology · 2021
    Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Sara D Siegel *Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston, Texas, USA.
Brendan R Amer *Department of Chemistry and Biochemistry and the UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, California, USA.
Chenggang WuDepartment of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston, Texas, USA.
Michael R SawayaDepartment of Chemistry and Biochemistry and the UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, California, USA.
Jason E GosschalkDepartment of Chemistry and Biochemistry and the UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, California, USA.
Robert T ClubbDepartment of Chemistry and Biochemistry and the UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, California, USA rclubb@mbi.ucla.edu htonthat@dentistry.ucla.edu.
Hung Ton-ThatDepartment of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston, Texas, USA rclubb@mbi.ucla.edu htonthat@dentistry.ucla.edu.ORCID 0000-0003-1611-0469
University of California, Los Angeles · USThe University of Texas Health Science Center at Houston · US

Funding

User Training and OutreachP30GM124165 · NIGMS · CORNELL UNIVERSITY · PI Nozomi Ando, J Christopher Fromme · 2018 to 2026
$34.2M
Cell Surface Protein Anchoring in Gram-Positive BacteriaR01AI052217 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Thompson Clubb · 2002 to 2026
$8.5M
Molecular Assembly on the Cell Surface of ActinomycesR01DE017382 · NIDCR · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI Hung Ton-That · 2008 to 2026
$6.7M
Post-translocational protein folding in Gram-positive bacteriaR01DE025015 · NIDCR · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI TON-THAT, HUNG · 2015 to 2024
$3.9M
Development of a cell-based assay to discover new bacterial anti-infective agentsR01AI121360 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CLUBB, ROBERT THOMPSON · 2016 to 2018
$1.1M
Mechanism of pilus hijacking and adherence by the coaggregation factor CafA in Actinomyces orisF31DE027295 · NIDCR · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI SIEGEL, SARA DANIELLE · 2017 to 2018
$41k
NIAID NIH HHS R01 AI052217NIAID NIH HHS R01 AI121360NIDCR NIH HHS F31 DE027295NIDCR NIH HHS R01 DE017382NIDCR NIH HHS R01 DE025015NIGMS NIH HHS P30 GM124165
6 · The paper itself

Abstract

The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope. However, it is undetermined if these enzymes are capable of catalyzing glycan attachment to surface proteins. In the actinobacterium

Indexed as

ActinomycesAmino Acid SubstitutionBacterial ProteinsCatalytic DomainCrystallography, X-RayDNA Mutational AnalysisGlycosylationHeat-Shock ProteinsModels, MolecularPhosphotransferasesProtein ConformationBacterial Proteinsglobal stress protein A, bacteriaHeat-Shock ProteinsPhosphotransferasescell wallGram-positive bacteriaLCPphosphotransferaseprotein foldingprotein glycosylationsortaseX-ray crystallography

Identifiers

PMID30782654
PMCPMC6381275
OpenAlexW2916629563

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.