ArticleInternational journal of molecular sciences2019
Exploration of miRNA and mRNA Profiles in Fresh and Frozen-Thawed Boar Sperm by Transcriptome and Small RNA Sequencing.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed.
- Sperm RNA landscape during sexual maturation in Duroc boars.BMC genomics · 2026Article
- Review
- Serum expression signature of TUG1, MALAT1, miR-483, and miR-141 and their targets TGF-β1 and STAT3 in severe male factor infertility.Scientific reports · 2025Article
- A comparison of the expression patterns and diagnostic capability of the ncRNAs NEAT1 and miR-34a in non-obstructive azoospermia and severe oligospermia.Human genomics · 2025Article
- Article
- Silent cells? Potential for context-dependent gene expression in mature sperm.Proceedings. Biological sciences · 2025Review
- RNA profiles differ between small and large extracellular vesicle subsets isolated from porcine seminal plasma.BMC genomics · 2024Article
- Article
- Effects of Slow Freezing and Vitrification of Human Semen on Post-Thaw Semen Quality and miRNA Expression.International journal of molecular sciences · 2024Article
- A Pilot Analysis of Whole Transcriptome of Human Cryopreserved Sperm.International journal of molecular sciences · 2024Article
- Long-term storage modifies the microRNA expression profile of cryopreserved human semen.Biomolecules & biomedicine · 2024Article
- Seminal plasma microbiomes, sperm parameters, and cryopreservation in a healthy fertile population.Frontiers in microbiology · 2024Article
- A review on the functional roles of trehalose during cryopreservation of small ruminant semen.Frontiers in veterinary science · 2024Review
- Effect of arginine-induced motility and capacitation on RNA population in goat spermatozoa.Veterinary research communications · 2023Article
- Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects.International journal of molecular sciences · 2023Review
- Seminal plasma metabolomics analysis of differences in liquid preservation ability of boar sperm.Journal of animal science · 2023Article
- Integrated transcriptomics and proteomics assay identifies the role of FCGR1A in maintaining sperm fertilization capacity during semen cryopreservation in sheep.Frontiers in cell and developmental biology · 2023Article
- Auto-immunoproteomics analysis of COVID-19 ICU patients revealed increased levels of autoantibodies related to the male reproductive system.Frontiers in physiology · 2023Article
- Sperm Transcriptome Analysis Accurately Reveals Male Fertility Potential in Livestock.Animals : an open access journal from MDPI · 2022Review
- Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology.Animals : an open access journal from MDPI · 2022Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Due to lower farrowing rate and reduced litter size with frozen-thawed semen, over 90% of artificial insemination (AI) is conducted using liquid stored boar semen. Although substantial progress has been made towards optimizing the cryopreservation protocols for boar sperm, the influencing factors and underlying mechanisms related to cryoinjury and freeze tolerance of boar sperm remain largely unknown. In this study, we report the differential expression of mRNAs and miRNAs between fresh and frozen-thawed boar sperm using high-throughput RNA sequencing. Our results showed that 567 mRNAs and 135 miRNAs were differentially expressed (DE) in fresh and frozen-thawed boar sperm. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the majority of DE mRNAs were enriched in environmental information processing such as cytokine-cytokine receptor interactions, PI3K-Akt signaling, cell adhesion, MAPK, and calcium signaling pathways. Moreover, the targets of DE miRNAs were enriched in significant GO terms such as cell process, protein binding, and response to stimuli. In conclusion, we speculate that DE mRNAs and miRNAs are heavily involved in boar sperm response to environment stimuli, apoptosis, and metabolic activities. The differences in expression also reflect the various structural and functional changes in sperm during cryopreservation.
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