ArticleMaterials (Basel, Switzerland)2019
PLA-Based Mineral-Doped Scaffolds Seeded with Human Periapical Cyst-Derived MSCs: A Promising Tool for Regenerative Healing in Dentistry.
Article in Materials (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 75 citations in OpenAlex.
- Biopolymers and Their Application in Bioprinting Processes for Dental Tissue Engineering.Pharmaceutics · 2023Review
- Application of Biocompatible Scaffolds in Stem-Cell-Based Dental Tissue Engineering.Advances in experimental medicine and biology · 2023Review
- Exploiting Dentine Matrix Proteins in Cell-Free Approaches for Periradicular Tissue Engineering.Tissue engineering. Part B, Reviews · 2022Review
- Biomaterials for Regenerative Medicine in Italy: Brief State of the Art of the Principal Research Centers.International journal of molecular sciences · 2022Review
- The healing of bone defects by cell-free and stem cell-seeded 3D-printed PLA tissue-engineered scaffolds.Journal of orthopaedic surgery and research · 2022Article
- Nanomaterials in Dentistry: Current Applications and Future Scope.Nanomaterials (Basel, Switzerland) · 2022Review
- Article
- In Vivo Study of Nasal Bone Reconstruction with Collagen, Elastin and Chitosan Membranes in Abstainer and Alcoholic Rats.Polymers · 2022Article
- Bone regeneration of induced pluripotent stem cells derived from peripheral blood cells in collagen sponge scaffolds.Journal of applied oral science : revista FOB · 2022Article
- Synthetic materials in craniofacial regenerative medicine: A comprehensive overview.Frontiers in bioengineering and biotechnology · 2022Review
- In vitro evaluation of periapical lesion-derived stem cells for dental pulp tissue engineering.FEBS open bio · 2022Article
- Rehabilitation of the Completely Edentulous Mandible by All-on-Four Treatment Concept: A Retrospective Cohort Study with Up to 10 Years Follow-Up.Medicina (Kaunas, Lithuania) · 2021Article
- Green Hydrogels Composed of Sodium Mannuronate/Guluronate, Gelatin and Biointeractive Calcium Silicates/Dicalcium Phosphate Dihydrate Designed for Oral Bone Defects Regeneration.Nanomaterials (Basel, Switzerland) · 2021Article
- Periodontal Bifunctional Biomaterials: Progress and Perspectives.Materials (Basel, Switzerland) · 2021Review
- Challenges and strategy in treatment with exosomes for cell-free-based tissue engineering in dentistry.Future science OA · 2021Review
- Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential.International journal of molecular sciences · 2021Review
- Stem Cells and Their Derivatives-Implications for Alveolar Bone Regeneration: A Comprehensive Review.International journal of molecular sciences · 2021Review
- Description of Joint Alterations Observed in a Family Carrying p.Asn453SerBiomolecules · 2021Article
- Interfacial Compatibilization into PLA/Mg Composites for Improved In Vitro Bioactivity and Stem Cell Adhesion.Molecules (Basel, Switzerland) · 2021Article
- Article
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Authors and funding
14 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human periapical cyst mesenchymal stem cells (hPCy-MSCs) are a newly discovered cell population innovatively collected from inflammatory periapical cysts. The use of this biological waste guarantees a source of stem cells without any impact on the surrounding healthy tissues, presenting a valuable potential in tissue engineering and regenerative medicine applications. In the present study, hPCy-MSCs were collected, isolated, and seeded on three experimental mineral-doped porous scaffolds produced by the thermally-induced phase-separation (TIPS) technique. Mineral-doped scaffolds, composed of polylactic acid (PLA), dicalcium phosphate dihydrate (DCPD), and/or hydraulic calcium silicate (CaSi), were produced by TIPS (PLA-10CaSi, PLA-5CaSi-5DCPD, PLA-10CaSi-10DCPD). Micro-CT analysis evaluated scaffolds micromorphology. Collected hPCy-MSCs, characterized by cytofluorimetry, were seeded on the scaffolds and tested for cell proliferation, cells viability, and gene expression for osteogenic and odontogenic differentiation (DMP-1, OSC, RUNX-2, HPRT). Micro-CT revealed an interconnected highly porous structure for all the scaffolds, similar total porosity with 99% open pores. Pore wall thickness increased with the percentage of CaSi and DCPD. Cells seeded on mineral-doped scaffolds showed a superior proliferation compared to pure PLA scaffolds (control), particularly on PLA-10CaSi-10DCPD at day 12. A higher number of non-viable (red stained) cells was observable on PLA scaffolds at days 14 and 21. DMP-1 expression increased in hPCy-MSCs cultured on all mineral-doped scaffolds, in particular on PLA-5CaSi-5DCPD and PLA-10CaSi-10DCPD. In conclusion, the innovative combination of experimental scaffolds colonized with autologous stem cells from periapical cyst represent a promising strategy for regenerative healing of periapical and alveolar bone.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.