Evidence map›Paper›PMID 30779922›Full record

ArticleGastroenterology2019

MicroRNA-31 Reduces Inflammatory Signaling and Promotes Regeneration in Colon Epithelium, and Delivery of Mimics in Microspheres Reduces Colitis in Mice.

Yuhua Tian, Jiuzhi Xu, Yuan Li, Ran Zhao, Sujuan Du, Cong Lv, Wei Wu, Ruiqi Liu, Xiaole Sheng, Yongli Song and 21 more

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Gastroenterology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
115citing papers in PubMed, 1 pooled it
8.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

115 citing papers in PubMed, 1 synthesis or guideline pooled it, 216 citations in OpenAlex.

  1. Pooled it
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  14. Radiation-sensitive circRNA promotes intestinal regeneration.Cellular & molecular biology letters · 2025
    Article
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55 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

31 authors at 13 institutions in 3 countries.

Yuhua TianState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Jiuzhi XuState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Yuan LiBeijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Food Sciences and Nutritional Engineering, China Agricultural University, Beijing, China.
Ran ZhaoState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Sujuan DuState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Cong LvState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Wei WuDepartment of Gastroenterology, The Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
Ruiqi LiuState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Xiaole ShengState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Yongli SongState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Xueyun BiState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Guilin LiState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Mengzhen LiState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Xi WuState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Pengbo LouState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China.
Huiwen YouSchool of Pharmaceutical Sciences, Capital Medical University, Beijing, China.
Wei CuiState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China; Institute of Reproductive and Developmental Biology, Faculty of Medicine, Imperial College London, UK.
Jinyue SunInstitute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, China.
Jianwei ShuaiDepartment of Physics and State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, Xiamen University, Xiamen, China.
Fazheng RenBeijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Food Sciences and Nutritional Engineering, China Agricultural University, Beijing, China.
Bing ZhangCollege of Veterinary Medicine, China Agricultural University, Beijing, China.
Mingzhou GuoDepartment of Gastroenterology and Hepatology, Chinese PLA General Hospital, Beijing, China.
Xiaohua HouDivision of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Technology and Science, Wuhan, China.
Kaichun WuDepartment of Gastroenterology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
Lixiang XueMedical Research Center. Department of Radiation Oncology, Peking University Third Hospital, Beijing, China.
Hongquan ZhangLaboratory of Molecular Cell Biology and Tumor Biology, Department of Anatomy, Histology and Embryology, Beijing, China.
Maksim V PlikusDepartment of Developmental and Cell Biology, Sue and Bill Gross Stem Cell Research Center, Center for Complex Biological Systems, University of California, Irvine, Irvine, California.
Yingzi CongDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas.
Christopher J LengnerDepartment of Biomedical Sciences, School of Veterinary Medicine, and Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
Zhanju LiuDepartment of Gastroenterology, The Shanghai Tenth People's Hospital, Tongji University, Shanghai, China. Electronic address: liuzhanju88@126.com.
Zhengquan YuState Key Laboratories for Agrobiotechnology and Beijing Advanced Innovation Center for Food Nutrition and Human Health and, College of Biological Sciences, China Agricultural University, Beijing, China. Electronic address: zyu@cau.edu.cn.
China Agricultural University · CNTongji University · CNCapital Medical University · CNChinese PLA General Hospital · CNImperial College London · GBPeking University · CNShandong Academy of Agricultural Sciences · CNThe University of Texas Medical Branch at Galveston · USUnion Hospital · CNUniversity of California, Irvine · USUniversity of Pennsylvania · USXiamen University · CNXijing Hospital · CN

Funding

The basis for and function of enteroendocrine lineage plasticity in the intestinal DNA damage responseR01DK106309 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI LENGNER, CHRISTOPHER JOACHIM · 2016 to 2023
$3.3M
6 · The paper itself

Abstract

BACKGROUND &

aimsLevels of microRNA 31 (MIR31) are increased in intestinal tissues from patients with inflammatory bowel diseases and colitis-associated neoplasias. We investigated the effects of this microRNA on intestinal inflammation by studying mice with colitis.

methodsWe obtained colon biopsy samples from 82 patients with ulcerative colitis (UC), 79 patients with Crohn's disease (CD), and 34 healthy individuals (controls) at Shanghai Tenth People's Hospital. MIR31- knockout mice and mice with conditional disruption of Mir31 specifically in the intestinal epithelium (MIR31 conditional knockouts) were given dextran sulfate sodium (DSS) or 2,4,6-trinitrobenzene sulfonic acid (TNBS) to induce colitis. We performed chromatin immunoprecipitation and luciferase assays to study proteins that regulate expression of MIR31, including STAT3 and p65, in LOVO colorectal cancer cells and organoids derived from mouse colon cells. Partially hydrolyzed alpha-lactalbumin was used to generate peptosome nanoparticles, and MIR31 mimics were loaded onto their surface using electrostatic adsorption. Peptosome-MIR31 mimic particles were encapsulated into oxidized konjac glucomannan (OKGM) microspheres, which were administered by enema into the large intestines of mice with DSS-induced colitis. Intestinal tissues were collected and analyzed by histology and immunohistochemistry.

resultsLevels of MIR31 were increased in inflamed mucosa from patients with CD or UC, and from mice with colitis, compared with controls. STAT3 and nuclear factor-κB activated transcription of MIR31 in colorectal cancer cells and organoids in response to tumor necrosis factor and interleukin (IL)6. MIR31-knockout and conditional-knockout mice developed more severe colitis in response to DSS and TNBS, with increased immune responses, compared with control mice. MIR31 bound to 3' untranslated regions of Il17ra and Il7r messenger RNAs (RNAs) (which encode receptors for the inflammatory cytokines IL17 and IL7) and Il6st mRNA (which encodes GP130, a cytokine signaling protein). These mRNAs and proteins were greater in MIR31-knockout mice with colitis, compared with control mice; MIR31 and MIR31 mimics inhibited their expression. MIR31 also promoted epithelial regeneration by regulating the WNT and Hippo signaling pathways. OKGM peptosome-MIR31 mimic microspheres localized to colonic epithelial cells in mice with colitis; they reduced the inflammatory response, increased body weight and colon length, and promoted epithelial cell proliferation.

conclusionsMIR31, increased in colon tissues from patients with CD or UC, reduces the inflammatory response in colon epithelium of mice by preventing expression of inflammatory cytokine receptors (Il7R and Il17RA) and signaling proteins (GP130). MIR31 also regulates the WNT and Hippo signaling pathways to promote epithelial regeneration following injury. OKGM peptosome-MIR31 microspheres localize to the colon epithelium of mice to reduce features of colitis. Transcript Profiling: GSE123556.

Indexed as

AnimalsBiomarkersBiopsy, NeedleCase-Control StudiesChinaColitis, UlcerativeCrohn DiseaseDisease Models, AnimalHumansImmunohistochemistryIntestinal MucosaMiceMice, KnockoutMicroRNAsMicrospheresRandom AllocationBiomarkersMicroRNAsMirn31 microRNA, mouseRNA, MessengerGene RegulationIBDNanoparticle Delivery SystemPosttranscriptional Processing

Identifiers

PMID30779922
OpenAlexW2911247714

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.