ArticleBiomacromolecules2019
Molecular Characterization of Mucus Binding.
Article in Biomacromolecules, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Understanding Aerosol-Mediated Disease Transmission.ACS central science · 2025Review
- Synthesis, Microbiology, and Biophysical Characterization of Mutanofactins from the Human Oral Microbiome.ACS central science · 2025Article
- Impact of Hydrophobic, Hydrophilic, and Mucus-Binding Motifs on the Therapeutic Potential of Ceftazidime Analogs for Pulmonary Administration.Antibiotics (Basel, Switzerland) · 2025Article
- Tracing the path of Quorum sensing molecules in cystic fibrosis mucus in a biomimetic in vitro permeability platform.Scientific reports · 2024Article
- Mucus polymer concentration andmBio · 2024Article
- Water Sorption and Structural Properties of Human Airway Mucus in Health and Muco-Obstructive Diseases.Biomacromolecules · 2024Article
- Comparative mucomic analysis of three functionally distinct Cornu aspersum Secretions.Nature communications · 2023Article
- Native gastrointestinal mucus: Critical features and techniques for studying interactions with drugs, drug carriers, and bacteria.Advanced drug delivery reviews · 2023Review
- Evolution of the Chronic Venous Leg Ulcer Microenvironment and Its Impact on Medical Devices and Wound Care Therapies.Journal of clinical medicine · 2023Review
- Mucus from human bronchial epithelial cultures: rheology and adhesion across length scales.Interface focus · 2022Article
- Mucosomes: Intrinsically Mucoadhesive Glycosylated Mucin Nanoparticles as Multi-Drug Delivery Platform.Advanced healthcare materials · 2022Article
- Molecular Dynamics Simulations to Explore the Structure and Rheological Properties of Normal and Hyperconcentrated Airway Mucus.Studies in applied mathematics (Cambridge, Mass.) · 2021Article
- Alkaline pH Increases Swimming Speed and Facilitates Mucus Penetration for Vibrio cholerae.Journal of bacteriology · 2021Article
- Home, sweet home: how mucus accommodates our microbiota.The FEBS journal · 2021Review
- Membrane-tethered mucin-like polypeptides sterically inhibit binding and slow fusion kinetics of influenza A virus.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Mucin Thin Layers: A Model for Mucus-Covered Tissues.International journal of molecular sciences · 2019Article
- Silencing glycosaminoglycan functions in mouse embryonic stem cells with small molecule antagonists.Methods in enzymology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Binding of small molecules to mucus membranes in the body has an important role in human health, as it can affect the diffusivity and activity of any molecule that acts in a mucosal environment. The binding of drugs and of toxins and signaling molecules from mucosal pathogens is of particular clinical interest. Despite the importance of mucus-small molecule binding, there is a lack of data revealing the precise chemical features of small molecules that lead to mucus binding. We developed a novel equilibrium dialysis assay to measure the binding of libraries of small molecules to mucin and other mucus components, substantially increasing the throughput of small molecule binding measurements. We validated the biological relevance of our approach by quantifying binding of the antibiotic colistin to mucin, and showing that this binding was associated with inhibition of colistin's bioactivity. We next used a small molecule microarray to identify 2,4-diaminopyrimidine as a mucin binding motif and confirmed the importance of this motif for mucin binding using equilibrium dialysis. Furthermore, we showed that, for molecules with this motif, binding to mucins and the mucus-associated biopolymers DNA and alginate is modulated by differences in hydrophobicity and charge. Finally, we showed that molecules lacking the motif exhibited different binding trends from those containing the motif. These results open up the prospect of routine testing of small molecule binding to mucus and optimization of drugs for clinically relevant mucus binding properties.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.