ArticleAMB Express2019
Enhancement of ascomycin production via a combination of atmospheric and room temperature plasma mutagenesis in Streptomyces hygroscopicus and medium optimization.
Article in AMB Express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 25 citations in OpenAlex.
- Optimized biosynthesis and performance enhancement of γ-PGA from Bacillus licheniformis: a study on wettability, microstructure, and environmental performance.Scientific reports · 2025Article
- Improving butenyl-spinosyn production inFrontiers in microbiology · 2025Article
- Article
- A High-Throughput Method Based on Microculture Technology for Screening of High-Yield Strains of Tylosin-ProducingJournal of microbiology and biotechnology · 2023Article
- Optimization of FK-506 production in Streptomyces tsukubaensis by modulation of Crp-mediated regulation.Applied microbiology and biotechnology · 2023Article
- Improvement of rimocidin production in Streptomyces rimosus M527 by reporter-guided mutation selection.Journal of industrial microbiology & biotechnology · 2023Article
- Development of a native-locus dual reporter system for the efficient screening of the hyper-production of natural products inFrontiers in bioengineering and biotechnology · 2023Article
- Avilamycin production enhancement by mutagenesis and fermentation optimization in Streptomyces viridochromogenes.World journal of microbiology & biotechnology · 2022Article
- Enhanced ascomycin production in Streptomyces hygroscopicus var. ascomyceticus by employing polyhydroxybutyrate as an intracellular carbon reservoir and optimizing carbon addition.Microbial cell factories · 2021Article
- Bioprocess and genetic engineering aspects of ascomycin production: a review.Journal, genetic engineering & biotechnology · 2020Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Ascomycin, a key intermediate for chemical synthesis of immunosuppressive drug pimecrolimus, is produced by Streptomyces hygroscopicus var. ascomyceticus. In order to improve the strain production, the original S. hygroscopicus ATCC 14891 strain was treated here with atmospheric and room temperature plasma to obtain a stable high-producing S. hygroscopicus SFK-36 strain which produced 495.3 mg/L ascomycin, a 32.5% increase in ascomycin compared to the ATCC 14891. Then, fermentation medium was optimized using response surface methodology to further enhance ascomycin production. In the optimized medium containing 81.0 g/L soluble starch, 57.4 g/L peanut meal, and 15.8 g/L soybean oil, the ascomycin yield reached 1466.3 mg/L in flask culture. Furthermore, the fermentation process was carried out in a 5 L fermenter, and the ascomycin yield reached 1476.9 mg/L, which is the highest ascomycin yield reported so far. Therefore, traditional mutagenesis breeding combined with medium optimization is an effective approach for the enhancement of ascomycin production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.