ArticleMolecular genetics and genomics : MGG2019
Exploring molecular tools for transformation and gene expression in the cultivated edible mushroom Agrocybe aegerita.
Article in Molecular genetics and genomics : MGG, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- The Transformation and Protein Expression of the Edible MushroomJournal of fungi (Basel, Switzerland) · 2025Article
- Development of Protoplast-Based Gene Editing System for Areca Palm.Plants (Basel, Switzerland) · 2025Article
- Towards engineering agaricomycete fungi for terpenoid production.Journal of industrial microbiology & biotechnology · 2024Review
- Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects.Journal of fungi (Basel, Switzerland) · 2024Review
- The Role of Chromatin and Transcriptional Control in the Formation of Sexual Fruiting Bodies in Fungi.Microbiology and molecular biology reviews : MMBR · 2022Review
- Organic Nitrogen Supplementation Increases Vegetative and Reproductive Biomass in a Versatile White Rot Fungus.Journal of fungi (Basel, Switzerland) · 2022Article
- Identification of Key Regulatory Pathways of Basidiocarp Formation inJournal of fungi (Basel, Switzerland) · 2022Article
- Resources of Iranian agarics (Basidiomycota) with an outlook on their antioxidant potential.Frontiers in microbiology · 2022Article
- The Pacific Tree-Parasitic FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Genetic Manipulation of the Brassicaceae Smut FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Identification and Expression of New Unspecific Peroxygenases - Recent Advances, Challenges and Opportunities.Frontiers in bioengineering and biotechnology · 2021Review
- A Novel Site-Specific Integration System for Genetic Modification ofG3 (Bethesda, Md.) · 2020Article
- Article
- Multilocus phylogeny- and fruiting feature-assisted delimitation of EuropeanMycological progress · 2020Article
- Stable and transient transformation, and a promoter assay in the selective lignin-degrading fungus, Ceriporiopsis subvermispora.AMB Express · 2019Article
- New terpenoids from the fermentation broth of the edible mushroomBeilstein journal of organic chemistry · 2019Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Agrocybe aegerita is a cultivated edible mushroom in numerous countries, which also serves as a model basidiomycete to study fruiting body formation. Aiming to create an easily expandable customised molecular toolset for transformation and constitutive gene of interest expression, we first created a homologous dominant marker for transformant selection. Progeny monokaryons of the genome-sequenced dikaryon A. aegerita AAE-3 used here were identified as sensitive to the systemic fungicide carboxin. We cloned the wild-type gene encoding the iron-sulphur protein subunit of succinate dehydrogenase AaeSdi1 including its up- and downstream regions, and introduced a single-point mutation (His237 to Leu) to make it confer carboxin resistance. PEG-mediated transformation of protoplasts derived from either oidia or vegetative monokaryotic mycelium with the resulting carboxin resistance marker (Cbx
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