ArticleThe Journal of antimicrobial chemotherapy2019
Silver nanoparticles present high intracellular and extracellular killing against Staphylococcus aureus.
Article in The Journal of antimicrobial chemotherapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The trial behind it
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Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
- Z‑Amino Acids Present Innovative Antimicrobial and Antibiofilm Properties against Methicillin-Susceptible and -ResistantACS omega · 2026Article
- Silver integrated hybrids and nanocomposites for next-generation biomedicine: Beyond antimicrobial coatings toward smart sense-response-heal platforms.Materials today. Bio · 2025Review
- Anti-Rickettsial Activity of Chitosan, Selenium, and Silver Nanoparticles: Efficacy in Vero Cells.Pathogens (Basel, Switzerland) · 2025Article
- Toxic Shock Syndrome Toxin-1 (TSST-1) inToxins · 2025Review
- Femtosecond Laser-Engineered β-TCP Scaffolds: A Comparative Study of Green-Synthesized AgNPs vs. Ion Doping AgainstInternational journal of molecular sciences · 2025Article
- A Comprehensive Review of Nanomaterials as Potential Weapons against Multidrug-resistantPharmaceutical nanotechnology · 2025Review
- Polyvinylpyrrolidone capped silver nanoparticles enhance the autophagic clearance of Acinetobacter baumannii from human pulmonary cells.Discover nano · 2024Article
- Multifaceted Assessment of Porous Silica Nanocomposites: Unraveling Physical, Structural, and Biological Transformations Induced by Microwave Field Modification.Nanomaterials (Basel, Switzerland) · 2024Article
- Simeprevir restores the anti-Staphylococcus activity of polymyxins.AMB Express · 2023Article
- The Impact of Silver Nanoparticle-Induced Photothermal Therapy and Its Augmentation of Hyperthermia on Breast Cancer Cells Harboring Intracellular Bacteria.Pharmaceutics · 2023Article
- Regulation ofPharmaceutics · 2023Review
- Ebselen Optimized the Therapeutic Effects of Silver Nanoparticles for Periodontal Treatment.International journal of nanomedicine · 2023Article
- Chemical Synthesis of Innovative Silver Nanohybrids with Synergistically Improved Antimicrobial Properties.International journal of nanomedicine · 2023Article
- Inhibition of the Vancomycin Resistance inBioMed research international · 2022Article
- Non-antibiotic strategies for prevention and treatment of internalizedFrontiers in microbiology · 2022Review
- Update on the role of antiseptics in the management of chronic wounds with critical colonisation and/or biofilm.International wound journal · 2021Review
- Inorganic nanohybrids combat antibiotic-resistant bacteria hiding within human macrophages.Nanoscale · 2021Article
- Silver Nanoparticles Induce aFrontiers in microbiology · 2021Article
- Hemostatic materials in wound care.Burns & trauma · 2021Review
- Mechanisms of Antibiotic Failure DuringFrontiers in immunology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
objectivesBone and joint infections caused by Staphylococcus aureus are becoming increasingly difficult to treat due to rising antibiotic resistance, resilient biofilms and intracellular survival of S. aureus. It has been challenging to identify and develop antimicrobial agents that can be used to kill extracellular and intracellular bacteria while having limited toxicity towards host cells. In addressing this challenge, this study investigates the antimicrobial efficacy and toxicity of silver nanoparticles (AgNPs).
methodsIntracellular bacteria were generated using a co-culture model of human osteoblast cells and S. aureus. Extracellular and intracellular S. aureus were treated with AgNPs, antibiotics and their combinations, and numbers of colonies were quantified. Toxicity of AgNPs against human osteoblast cells was determined by quantifying the number of viable cells after treatment.
resultsAgNPs demonstrated excellent antimicrobial activity against extracellular S. aureus with a 100% killing efficacy at concentrations as low as 56 μM, along with a high intracellular killing efficacy of 76% at 371 μM. AgNPs were non-toxic or slightly toxic towards human osteoblasts at the concentrations studied (up to 927 μM). Moreover, smaller-sized (40 nm) AgNPs were more efficacious in killing bacteria compared with their larger-sized (100 nm) counterparts and synergistic antimicrobial effects against extracellular bacteria were observed when AgNPs were combined with gentamicin.
conclusionsAgNPs and their combination with antibiotics have demonstrated high extracellular and intracellular bacterial killing and presented unique aspects for potential clinical applications, especially for chronic and recurrent infections where intracellular bacteria may be the cause.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.