ArticleBiomaterials science2019
Spatiotemporal control and modeling of morphogen delivery to induce gradient patterning of stem cell differentiation using fluidic channels.
Article in Biomaterials science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Bioactive photo-crosslinkable hydrogel based on decellularized dental pulp matrix for functional pulp regeneration.BMC oral health · 2026Article
- Modeling development using hydrogels.Development (Cambridge, England) · 2023Article
- Engineering Smart Composite Hydrogels for Wearable Disease Monitoring.Nano-micro letters · 2023Review
- Synthetic developmental biology: Engineering approaches to guide multicellular organization.Stem cell reports · 2022Review
- Insights into the present and future of cartilage regeneration and joint repair.Cell regeneration (London, England) · 2022Review
- A Progress Report and Roadmap for Microphysiological Systems and Organ-On-A-Chip Technologies to Be More Predictive Models in Human (Knee) Osteoarthritis.Frontiers in bioengineering and biotechnology · 2022Review
- Advances in neural organoid systems and their application in neurotoxicity testing of environmental chemicals.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Review
- Patterning Neuroepithelial Cell SheetACS biomaterials science & engineering · 2021Article
- Tissue Chips and Microphysiological Systems for Disease Modeling and Drug Testing.Micromachines · 2021Review
- Gradient Hydrogels.Advances in biochemical engineering/biotechnology · 2021Article
- Recent Advancements in Engineering Strategies for Manipulating Neural Stem Cell Behavior.Current tissue microenvironment reports · 2020Article
- BCNNM: A Framework forFrontiers in computational neuroscience · 2020Article
- Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three-Dimensional Hydrogels.Current protocols in stem cell biology · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The process of cell differentiation in a developing embryo is influenced by numerous factors, including various biological molecules whose presentation varies dramatically over space and time. These morphogens regulate cell fate based on concentration profiles, thus creating discrete populations of cells and ultimately generating large, complex tissues and organs. Recently, several in vitro platforms have attempted to recapitulate the complex presentation of extrinsic signals found in nature. However, it has been a challenge to design versatile platforms that can dynamically control morphogen gradients over extended periods of time. To address some of these issues, we introduce a platform using channels patterned in hydrogels to deliver multiple morphogens to cells in a 3D scaffold, thus creating a spectrum of cell phenotypes based on the resultant morphogen gradients. The diffusion coefficient of a common small molecule morphogen, retinoic acid (RA), was measured within our hydrogel platform using Raman spectroscopy and its diffusion in our platform's geometry was modeled using finite element analysis. The predictive model of spatial gradients was validated in a cell-free hydrogel, and temporal control of morphogen gradients was then demonstrated using a reporter cell line that expresses green fluorescent protein in the presence of RA. Finally, the utility of this approach for regulating cell phenotype was demonstrated by generating opposing morphogen gradients to create a spectrum of mesenchymal stem cell differentiation states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.