ArticleMaterials today. Chemistry2019
Cryopreserved cell-laden alginate microgel bioink for 3D bioprinting of living tissues.
Article in Materials today. Chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Advances in Engineering Human Tissue Models.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Cryopreservative Bioink Enables Direct Bioprinting of Adherent Cells.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Granular Hydrogels as Modular Biomaterials: From Structural Design to Biological Responses.Advanced healthcare materials · 2026Review
- TYRAY-Functionalized Alginate Bioinks for 3D Bioprinting Support Stem Cell Culture and Endothelial Network Formation.ACS biomaterials science & engineering · 2025Article
- A versatile and efficient method to quantify purity and degree of substitution in alginate derivatives.Carbohydrate polymers · 2025Article
- Recent Advances in Stimuli-Responsive Microgels and Their Biomedical Applications.Molecules (Basel, Switzerland) · 2025Review
- Practical Guide to the Design of Granular Hydrogels for Customizing Complex Cellular Microenvironments.Advanced healthcare materials · 2025Review
- Advances in Cryopreservation Strategies for 3D Biofabricated Constructs: From Hydrogels to Bioprinted Tissues.International journal of molecular sciences · 2025Review
- 3D-Printable Granular Hydrogel Composed of Hyaluronic Acid-Chitosan Hybrid Polyelectrolyte Complex Microgels.Biomacromolecules · 2025Article
- Microgel-based bioink for extrusion-based 3D bioprinting and its applications in tissue engineering.Bioactive materials · 2025Review
- Engineered Living Systems Based on Gelatin: Design, Manufacturing, and Applications.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Insights on the role of cryoprotectants in enhancing the properties of bioinks required for cryobioprinting of biological constructs.Journal of materials science. Materials in medicine · 2025Article
- Cutting-edge 3D printing in immunosensor design for early cancer detection.Mikrochimica acta · 2024Review
- Design approaches for 3D cell culture and 3D bioprinting platforms.Biophysics reviews · 2024Review
- Article
- DeepFreeze 3D-biofabrication for Bioengineering and Storage of Stem Cells in Thick and Large-Scale Human Tissue Analogs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Emerging granular hydrogel bioinks to improve biological function in bioprinted constructs.Trends in biotechnology · 2024Review
- The challenges and prospects of smooth muscle tissue engineering.Regenerative medicine · 2024Review
- Multiscale embedded printing of engineered human tissue and organ equivalents.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Cell-laden microgels have been used as tissue building blocks to create three-dimensional (3D) tissues and organs. However, traditional assembly methods can not be used to fabricate functional tissue constructs with biomechanical and structural complexity. In this study, we present directed assembly of cell-laden dual-crosslinkable alginate microgels comprised of oxidized and methacrylated alginate (OMA). Cell-laden OMA microgels can be directly assembled into well-defined 3D shapes and structures under low-level ultraviolet light. Stem cell-laden OMA microgels can be successfully cryopreserved for long-term storage and on-demand applications, and the recovered encapsulated cells maintained equivalent viability and functionality to the freshly processed stem cells. Finally, we have successfully demonstrated that cell-laden microgels can be assembled into complicated 3D tissue structures via freeform reversible embedding of suspended hydrogels (FRESH) 3D bioprinting. This highly innovative bottom-up strategy using FRESH 3D bioprinting of cell-laden OMA microgels, which are cryopreservable, provides a powerful and highly scalable tool for fabrication of customized and biomimetic 3D tissue constructs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.