ArticleNature plants2019
High-efficiency generation of fertile transplastomic Arabidopsis plants.
Article in Nature plants, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Editing and synthetic engineering of chloroplast genomes.Plant communications · 2026Review
- Plant- and Microalgae-Based Biotechnological Strategies for Affordable and Non-Invasive Delivery of Antidiabetic Peptides.Pharmaceutics · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Chloroplast genome comparative analysis and phylogenetic relationships of 15 Syringa species (Oleaceae).Genetics and molecular biology · 2026Article
- A modular high-throughput approach for advancing synthetic biology in the chloroplast of Chlamydomonas.Nature plants · 2025Article
- Development of chloroplast transformation for five species in the genus Nicotiana.The Plant journal : for cell and molecular biology · 2025Article
- Article
- Structure, regulation and assembly of the photosynthetic electron transport chain.Nature reviews. Molecular cell biology · 2025Review
- The trehalose 6-phosphate pathway coordinates dynamic changes at the shoot apical meristem in Arabidopsis thaliana.Plant physiology · 2025Article
- Core biological principles and tools stemming from basic Arabidopsis research.The Plant cell · 2025Review
- The lumenal domain of Cyt bPhotosynthesis research · 2025Article
- Suppression of plastid-to-nucleus gene transfer by DNA double-strand break repair.Nature plants · 2025Article
- Reverse genetics in the Arabidopsis chloroplast genome identifies rps16 as a transcribed pseudogene.The Plant journal : for cell and molecular biology · 2025Article
- Stable plastid transformation in kiwifruit (aBIOTECH · 2025Article
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Targeted C-to-T Base Editing in the Arabidopsis Plastid Genome.Current protocols · 2025Article
- Species-wide inventory ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform.ACS synthetic biology · 2024Article
- Comparative Chloroplast Genomes Analysis Provided Adaptive Evolution Insights inInternational journal of molecular sciences · 2024Article
- TALE-based organellar genome editing and gene expression in plants.Plant cell reports · 2024Review
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The development of technologies for the stable genetic transformation of plastid (chloroplast) genomes has been a boon to both basic and applied research. However, extension of the transplastomic technology to major crops and model plants has proven extremely challenging, and the species range of plastid transformation is still very much limited in that most species currently remain recalcitrant to plastid genome engineering. Here, we report an efficient plastid transformation technology for the model plant Arabidopsis thaliana that relies on root-derived microcalli as a source tissue for biolistic transformation. The method produces fertile transplastomic plants at high frequency when combined with a clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9)-generated knockout allele of a nuclear locus that enhances sensitivity to the selection agent used for isolation of transplastomic events. Our work makes the model organism of plant biology amenable to routine engineering of the plastid genome, facilitates the combination of plastid engineering with the power of Arabidopsis nuclear genetics, and informs the future development of plastid transformation protocols for other recalcitrant species.
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