Evidence map›Paper›PMID 30778111›Full record

ArticleScientific reports2019

Immobilization of Peroxidase on Functionalized MWCNTs-Buckypaper/Polyvinyl alcohol Nanocomposite Membrane.

Lau Yien Jun, N M Mubarak, Lau Sie Yon, Chua Han Bing, Mohammad Khalid, Priyanka Jagadish, E C Abdullah

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
5.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 90 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Lau Yien JunDepartment of Chemical Engineering, Faculty of Engineering and Science, Curtin University, 98009, Sarawak, Malaysia.
N M MubarakDepartment of Chemical Engineering, Faculty of Engineering and Science, Curtin University, 98009, Sarawak, Malaysia. mubarak.mujawar@curtin.edu.my.ORCID 0000-0001-7083-2521
Lau Sie YonDepartment of Chemical Engineering, Faculty of Engineering and Science, Curtin University, 98009, Sarawak, Malaysia.
Chua Han BingDepartment of Chemical Engineering, Faculty of Engineering and Science, Curtin University, 98009, Sarawak, Malaysia.
Mohammad KhalidGraphene & Advanced 2D Materials Research Group (GAMRG), School of Science and Technology, Sunway University, No. 5, Jalan University, Bandar Sunway, 47500, Subang Jaya, Selangor, Malaysia.
Priyanka JagadishGraphene & Advanced 2D Materials Research Group (GAMRG), School of Science and Technology, Sunway University, No. 5, Jalan University, Bandar Sunway, 47500, Subang Jaya, Selangor, Malaysia.
E C AbdullahDepartment of Chemical Process Engineering, Malaysia-Japan International Institute of Technology (MJIIT) Universiti Teknologi Malaysia (UTM), Jalan Sultan Yahya Petra, 54100, Kuala Lumpur, Malaysia.ORCID 0000-0002-4547-8046
Curtin University Sarawak · MYSunway University · MYUniversity of Technology Malaysia · MY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Surface modified Multi-walled carbon nanotubes (MWCNTs) Buckypaper/Polyvinyl Alcohol (BP/PVA) composite membrane was synthesized and utilized as support material for immobilization of Jicama peroxidase (JP). JP was successfully immobilized on the BP/PVA membrane via covalent bonding by using glutaraldehyde. The immobilization efficiency was optimized using response surface methodology (RSM) with the face-centered central composite design (FCCCD) model. The optimum enzyme immobilization efficiency was achieved at pH 6, with initial enzyme loading of 0.13 U/mL and immobilization time of 130 min. The results of BP/PVA membrane showed excellent performance in immobilization of JP with high enzyme loading of 217 mg/g and immobilization efficiency of 81.74%. The immobilized system exhibited significantly improved operational stability under various parameters, such as pH, temperature, thermal and storage stabilities when compared with free enzyme. The effective binding of peroxidase on the surface of the BP/PVA membrane was evaluated and confirmed by Field emission scanning electron microscopy (FESEM) coupled with Energy Dispersive X-Ray Spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric Analysis (TGA). This work reports the characterization results and performances of the surface modified BP/PVA membrane for peroxidase immobilization. The superior properties of JP-immobilized BP/PVA membrane make it promising new-generation nanomaterials for industrial applications.

Indexed as

Enzymes, ImmobilizedMembranes, ArtificialNanocompositesNanotubes, CarbonPolyvinyl AlcoholEnzyme ActivationEnzyme StabilityHydrogen-Ion ConcentrationPeroxidaseReproducibility of ResultsSpectrum AnalysisTemperatureThermodynamicsEnzymes, ImmobilizedMembranes, ArtificialNanotubes, CarbonPeroxidasePolyvinyl Alcohol

Identifiers

PMID30778111
PMCPMC6379398
OpenAlexW2913381691

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.